Reverse transcription

Reverse transcription (RT) is a widely used application in molecular biology research for RNA and expression analysis. The Thermo Scientific reverse transcription portfolio offers robust and reliable reverse transcriptases in a variety of optimized formats for efficiency in RT-PCR and RT-qPCR applications.

Thermo Scientific reverse transcription products

Selecting the right RT for cDNA synthesis is critical to detecting low-abundance RNAs or dealing with RNA that contains high amounts of secondary structure. Considerations for selecting the RT should include sensitivity and thermostability (ability to perform at higher temperature).

Stand-alone enzymes engineered for optimal cDNA synthesis

Enhanced performance Routine performance
Product Maxima H Minus Reverse Transcriptase Maxima Reverse Transcriptase RevertAid H Minus Reverse Transcriptase RevertAid Reverse Transcriptase
Active temperature range 50–65 oC 50–65 oC 42–55 o 42–50 oC

RNase H activity

No Yes No Yes
Reaction time 15-30 min 15-30 min 60 min 60 min

Sensitivity (total RNA)

1 pg 1 pg 0.1 ng 0.1 ng

Maximum cDNA length

20 kb 20 kb 13 kb 13 kb

Complete kits for efficient and reproducible first strand cDNA synthesis

Enhanced performance Routine performance
Product Maxima H Minus First Strand cDNA Synthesis Master Mixes Maxima First Strand cDNA Synthesis Kit RevertAid H Minus First Strand cDNA Synthesis Kit RevertAid First Strand cDNA Synthesis Kit
Active temperature range 50–65 oC 50–65 oC 42–55 o 42–50 oC

RNase H activity

No Yes No Yes
Reaction time 15-30 min 15-30 min 60 min 60 min
Formats with dsDNase* Yes Yes N/A N/A

Sensitivity (total RNA)

1 pg 1 pg 0.1 ng 0.1 ng

Maximum cDNA length

20 kb 20 kb 13 kb 13 kb
*dsDNase step is a quick (2 min) and easy gDNA removal step prior to RT step.

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Tailor-made performance tested products with strict quality standards to meet your molecular assay requirements