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qPCR assays and master mixes
Explore qPCR assays and master mixes based on your target, application, chemistry, and workflow.
qPCR assays and master mixes by research application
Access qPCR products and resources based on your application.
Why choose Applied Biosystems for qPCR?
With 30 years of qPCR innovation, Applied Biosystems offers assays, master mixes, tools, and resources to support qPCR research from assay and reagent selection through data analysis.
Featured qPCR insights
Read perspectives and practical guidance on the choices and approaches shaping qPCR research.
qPCR tools and resources
Find tools and guidance for selecting qPCR components, comparing detection chemistries, planning experiments, analyzing data, and troubleshooting common qPCR issues.
Understand key differences between probe-based TaqMan and dye-based SYBR Green detection and considerations for choosing a chemistry.
Access resources covering qPCR experimental design, reaction setup, data analysis, and other experimental considerations, including the qPCR Handbook, videos, FAQs, and educational guidance.
Find getting-started guidance, troubleshooting, guides and tools for custom TaqMan primers and probes.
Find guidance for common amplification, assay, reagent, and experimental issues.
Frequently asked questions (FAQs)
TaqMan Assays can be used with compatible probe-based qPCR master mixes. Master-mix selection may depend on the application, sample type, multiplexing requirements, cycling conditions, and real-time PCR instrument. Review the recommended master mixes and compatibility information for the assay and intended experimental conditions.
Primers are short DNA sequences that define the region amplified during qPCR. In probe-based qPCR, a target-specific fluorescent probe helps provide an additional detection mechanism for monitoring amplification. TaqMan Assays combine target-specific primers and probes in predesigned or custom assay formats for a range of research applications. Lab professionals who need custom sequences can use the Custom TaqMan Probes and Primers tool to enter or upload primer and probe sequences and select dyes and quenchers.
Predesigned qPCR assays can provide a convenient option when an assay is available for the target of interest. A custom assay may be considered when a suitable predesigned assay is unavailable or when research requires a specific target, sequence, or assay design. Use the Custom TaqMan Assay design tool to access custom options for gene expression, SNP genotyping, copy number, small RNA, probes, and primers.
ROX is a passive reference dye used with some qPCR master mixes and real-time PCR instruments to normalize non-PCR-related fluorescence variation. Whether a passive reference dye is required, and which dye is appropriate, depends on the master mix and instrument. Check the reagent and instrument compatibility information before selecting a master mix.
When planning a multiplex experiment, consider the number of targets, compatible reporter dyes, assay design, master mix, sample type, and real-time PCR instrument. Master mix and assay requirements can vary based on the level of multiplexing and application. Thermo Fisher Scientific’s TaqMan Multiplex Master Mix guidance helps provide recommendations based on the number of assays, application, instrument compatibility, and passive reference dye requirements. Learn more about TaqMan Multiplex solutions for qPCR.
Compatibility of Applied Biosystems qPCR master mixes with real-time PCR instruments depends on the master mix and instrument configuration. When evaluating compatibility, researchers may need to consider reaction volume, cycling conditions, fluorophore compatibility, and passive reference dye settings. Review the real-time PCR master mix and instrument compatibility guide for platform-specific information.
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For Research Use Only. Not for use in diagnostic procedures.