Search

  • Order Status
  • Quick Order

Selectris and Selectris X Imaging Filters

Cryo-EM imaging enhanced with zero-loss energy filtering for atomic-resolution cryo-EM

Available on Thermo Scientific Krios and Glacios Cryo-TEMs, Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of transmission electron microscopy (TEM) images, resulting in high-resolution structures up to atomic resolution. The zero-loss filtering of Selectris Filters removes noise caused by inelastically scattered electrons, producing an increased signal-to-noise ratio (SNR) and better contrast. Designed for high stability, the zero-loss peak position of Selectris Filters has minimized sensitivity to temperature variations in the environment, eliminating the need for frequent tuning.

Frequently asked questions about the Selectris Imaging Filters

Thermo Scientific Selectris and Selectris X Imaging Filters are post-column energy filters for cryo-transmission electron microscopy. They use zero-loss filtering to remove inelastically scattered electrons from the image-forming signal, improving image contrast and signal-to-noise ratio in cryo-EM data.

 

This is especially valuable for relatively thick cryo-electron tomography samples as well as single particle analysis, where increased contrast can support imaging closer to focus and improve the quality of data used for high-resolution reconstruction. The Selectris Filters are built for high stability and ease of use, helping you obtain high-resolution structures quickly for novel biological insights.

The Selectris X Imaging Filter builds on the Selectris platform with a more advanced electron-optical design for further aberration correction. This reduces distortion in both the image and energy domains, supporting demanding cryo-EM applications such as high-resolution/atomic-resolution single particle analysis. The Selectris Imaging Filter provides the same core benefits of zero-loss filtering, stability, and ease of use for high-performance cryo-EM workflows.

Vitrified biological samples are radiation sensitive, so cryo-EM data must be acquired under low-dose conditions. This limits the signal available in each exposure and makes image contrast and signal-to-noise ratio critical. Selectris Imaging Filters improve contrast by using zero-loss filtering to remove inelastically scattered electrons from the image-forming signal. This helps improve the signal-to-noise ratio of images, supports high-resolution reconstruction, and can increase the fraction of usable data. As a result, Selectris Imaging Filters can improve productivity across both single particle analysis and cryo-electron tomography workflows.

A Selectris Imaging Filter improves the signal before it reaches the detector by removing inelastically scattered electrons from the image-forming signal. The Thermo Scientific Falcon 4i Direct Electron Detector then quickly records the filtered signal with high DQE using accurate electron-event localization in the instrument’s native EER file format. Together, the Selectris Imaging Filter and Falcon 4i Detector help you extract more useful signal from challenging cryo-ET samples and support demanding high-resolution single particle analysis workflows.

Selectris Imaging Filters are available with new Thermo Scientific Krios and Glacios Cryo-TEMs. Retrofit options may also be available for many Thermo Scientific cryo-TEMs operating with Windows 10. For upgrades of existing systems, compatibility should be confirmed for the specific microscope configuration. 

For Research Use Only. Not for use in diagnostic procedures.