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Molecular Probes® Education Series Online Exam

Research Area Interests *

 Cancer
 Cell Cycle
 Cell Proliferation
 Cell Surface Markers
 Fluorescent Proteins
 Infectious Diseases
 Immunoassays
 Immunophenotyping
 Live Cell Imaging
 Neuroscience
 Protein Labeling
 Secondary Detection
 Subcellular Structural Analysis
 Toxicology

Contact Information

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Mailing Address

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Job Role

 CEO / COO / President
 Vice President
 Department Head
 Principal Investigator
 Medical Doctor
 Director
 Post-doctoral fellow
 Scientist / Associate Scientist
 Student / Graduate Student
 Research Assistant / Lab Technician
 Lab Manager
 Purchaser / Procurer
 Educator

Techniques

 Mass Spectrometry
 Cell Imaging
 qPCR / Real-time PCR / qRT-PCR
 DNA Sequencing
 Next Generation DNA Sequencing
 Nucleic Acid Purification and/or Separation
 Transfection
 Virus Isolation

Applications

 BioMarker Discovery
 Cell / Tissue Culture
 Cloning
 Environmental Testing
 Epigenetics / Epigenomics Analysis
 Food and Beverage Testing
 Forensics / Human Identity
 Gene Expression / RNA Analysis
 Genotyping and Genetic Variation
 Imaging & Microscopy
 In Vivo Research
 Plant Research
 Protein Analysis / Proteomics
 Protein Expression / Production
 RNAi
 Stem Cells

Q1. Which blocking reagents are suitable for immunocytochemistry? *

 BSA/serum
 PBS buffer
 No blocking needed
 Detergent Triton X-100
 Formaldehyde
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 True
 False
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 No secondary antibody
 No fixation
 No blocking
 No primary antibody
 No cells
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 True
 False
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 Reduce light exposure
 Choose a more photostable dye
 Use an anti-fade mounting medium
 All of the above
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 True
 False
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 Organic dyes
 Fluorescent proteins
 Quantum dots
 Colorimetric dyes
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 True
 False

Q4. Which of the following is an advantage to fluorescent proteins? *

 Photostability
 Ability to make bioconjugates
 High transduction efficiency
 Good for live cell imaging
 Not many color options
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 True
 False
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 True
 False
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 pH 4
 < pH 7
 pH 7.2
 pH 8-8.5
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 BSA
 Tris buffered saline
 Glycerol
 Azide
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 True
 False

Q6. Which reactive chemistry requires denaturing of the protein? *

 Amine
 Thiol
 EDAC crosslinking
 Click Chemistry
 Secondary antibodies
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 True
 False

Q8. Which kit/technology is best if my antibody contains a protein stabilizer like BSA? *

 APEX Antibody Labeling Kit
 Microscale protein labeling kit
 Alexa Fluor protein labeling kit
 Qdot Antibody Conjugation Kit
 Monoclonal Antibody Labeling Kit