Enzyme and Biotin-Labeled Secondary Antibodies
We offer both high-activity horseradish peroxidase and alkaline phosphatase secondary antibody conjugates for western blots, ELISA, immunohistochemistry, or other enzyme-driven applications.
We also offer a wide variety of biotin and DSB biotin conjugates. Utilizing a biotin-conjugated secondary antibody allows you to use the same secondary antibody in multiple applications, simply by switching the streptavidin being used.
Enzyme conjugates are used in conjunction with a substrate, whether colorimetric, fluorogenic, or chemiluminescent to create a detectable signal. The substrate is converted from a non-detectable soluble form to a detectable usually insoluble form. Since the enzyme can convert many molecules of substrate, this is a form of signal amplification controlled by the length of time the reaction is allowed to continue.
Biotin is a small molecule that can be bound by avidins, streptavidin, and NeutrAvidin with high affinity. Utilizing a biotin-conjugated secondary antibody allows you to use the same secondary antibody in multiple applications, simply by switching the streptavidin being used. Biotin/streptavidin strategies can also be employed for signal enhancement.
How to find a secondary antibody
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
We offer a wide selection of ELISA kits targeted to disease-related proteins for research in immunology, cancer, neurodegeneration, stem cells, and more.
Western blot reagents and instruments, chemiluminescent, colormetric, and fluorescent detection substrates, blocking buffers, and secondary antibody conjugates
We provide a complete suite of labeling tools for the reliable detection of many levels of target abundance. Our fluorescent products portfolio is highly cited in a wide range of life science research applications. Review this simple guide and learn which tool can help with your experiment.
We offer a wide variety of primary antibodies against key signaling proteins, cellular markers, and organelles, as well as cell junctions and CD markers. Primary antibodies provide direct detection for high-abundance targets, and high target specificity when using amplified detection methods.
Partner with us to scale-up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications.
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For Research Use Only. Not for use in diagnostic procedures.