Specificity of anti-RFP rabbit polyclonal antibody in immunocytochemistry (ICC). Following fixation and permeabilization, HeLa cells were stained with Invitrogen Alexa Fluor 350 phalloidin and anti-RFP rabbit polyclonal antibody, the latter subsequently visualized with Invitrogen Alexa Fluor 647 Goat Anti-Rabbit IgG antibody, red fluorescence and imaged. No cross-reactivity is observed of the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).
Red Fluorescent Protein (RFP)
Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
We offer a series of Invitrogen CellLight RFP fusion constructs of signal peptides or cell structure proteins with tagRFP for accurate and specific targeting to subcellular structures, including the cytoskeleton, mitochondria, and secretory compartments.
To complement these tools, we also offer anti-RFP antibodies and antibody conjugates for a wide variety of applications, including imaging, western blotting, and flow cytometry. All of our anti-RFP antibodies are suited for detection of native RFP, RFP variants, and CellLight fusion proteins.
|RFP is a useful expression label for flow cytometry or imaging applications. Weak signals can be retrieved or amplified using an anti-RFP antibody.|
Photostability in buffer
Photostability in antifade
Invitrogen CellLight reagents are ready-to-use fluorescent protein constructs targeted to specific subcellular structures. These reagents provide a simple and effective method for introducing targeted intracellular labels within living cells. Simply add the reagent to your cells, incubate overnight, and you’re ready to image your cells.
Learn more about CellLight reagents
Anti-RFP antibodies provide a convenient method for visualizing RFP, especially when amplification of the fluorescent protein of interest is necessary to overcome a dim or degraded signal. Our anti-RFP antibodies are easily incorporated into standard immunostaining protocols for cell and tissue analysis.
- Efficient transduction of cell lines, including primary cells and stem cells
- Safe transduction and lack of observable cytopathic effects
- Easy modulation of gene expression
- Expression of multi-component complexes at the right stoichiometry
The Invitrogen BacMam technology is based on double-stranded DNA insect viruses (baculovirus) as vehicles to efficiently deliver and express genes in mammalian cells, including those encoding fluorescent proteins, such as RFP. Baculoviruses are well-tolerated and nonreplicating in mammalian cells, so they have an excellent safety profile for routine applications.
Learn more about:
Live-cell imaging of HeLa cells using CellLight mitochondria and talin probes, transduced using BacMam 2.0 technology.
Molecular Probes Handbook
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.