Gibco Water for Injection for Bioprocessing

Quality WFI for cell culture for all your upstream and downstream bioprocessing needs

  • USP/EP Water for Injection
  • Manufactured in ISO certified, FDA registered, cGMP facilities
  • Sterile filtered to 0.1 µm

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Gibco Water for Injection (WFI) for Cell Culture is high-quality, cell culture–grade water that complies with both United States Pharmacopoeia (USP) and European Pharmacopoeia (EP) monographs for water for injection packaged in bulk for commercial use elsewhere. It is manufactured in ISO certified, cGMP, and FDA registered facilities. It meets the highest of standards in accordance with the biopharmaceutical, pharmaceutical, and diagnostic industries. 

Gibco Water for Injection (WFI) for Cell Culture is membrane filtered to 0.1 µm. It has a 24 month shelf life and extended storage temperature of 2–30°C. Every batch is produced using a vapor compression distillation method and undergoes strict quality control testing, including: 

  • Sterility
  • Endotoxin
  • Conductivity
  • Osmolality
  • Oxidizable substances
  • pH

Gibco WFI for cell culture meets the USP/EP monograph for water for injection at point of fill, packaged in bulk for commercial use elsewhere.

The Certificate of Analysis provides the following parameters:

Parameter Limits Units
Endotoxin Testing ≤0.25 EU/mL
Conductivity Measurement <1.0 us/cm
Osmolality ≥0 to ≤20 mOsm/Kg
Oxidizable Substances Meets USP requirements  
pH ≥4.0 to ≤7.5  
Sterility Testing Negative  
Gibco-WFI-230x195

Purity and quality are built into our Water for Injection production process

Our purification process produces WFI for cell culture of high quality, prepared by distillation and filtration (0.1 µm), with no added substances. Water quality is monitored on a routine basis; conductivity, total organic carbon, endotoxins, and bioburden are tested daily; metals are tested monthly.

Applications of WFI for Cell Culture

Water for Injection (WFI) for Cell Culture can be used for a wide variety of upstream and downstream process solutions.

Upstream

  • Cell culture media
  • Cell suspension and wash solutions
  • Formulating solutions
  • Sanitization of laboratory glassware and containers
  • Reconstitution of products, such as dry powder media

Downstream

  • Diluting buffers
  • Process buffers
  • Purification buffers
  • Cleaning solutions and rinse agents

For further manufacturing or research use. Not for diagnostic or therapeutic use.


To order new WFI for cell culture catalog offerings in 20 L and 200 L sizes, please call our customer care team at 1-800-955-6288 or email us at customerservice@thermofisher.com for orders in the United States.