Human Protein Microarrays Overview
Figure 1. (Left) The high-content human ProtoArray® Human Protein Microarray contains thousands of full-length human proteins for rapid profiling and easy biomarker discovery. (Right) The ProtoArray® Prospector software is a user-friendly tool that enables rapid data analysis and cross-group comparisons.
Absolute identification of your protein targets with ProtoArray® Human Protein Microarray
Each ProtoArray® protein array has over 9,000 human proteins printed in duplicate on a one inch by three inch slide. The proteins are produced from clones selected from the Invitrogen Ultimate™ ORF Collection, the first and only clone resource to offer full-insert sequenced clones in a Gateway® vector. Proteins are expressed as N-terminal GST fusion proteins using a baculovirus expression system. After protein expression, proteins are purified under nondenaturing conditions. The proteins are printed at +4°C on an ultrathin layer of nitrocellulose to preserve native protein structure without the need for chemical fixation. Each slide is labeled with a unique bar code for easy tracking.
Individual microarrays on nitrocellulose-coated glass slides allow for the greatest flexibility in experimental design, with protocols for each supported application available. Some of these profiling applications (Figure 2) include:
- Immune response biomarker profiling—biomarker discovery for rapid discovery of disease markers specific to a variety of infectious diseases, cancers, and autoimmune diseases
- Enzyme substrate profiling—profiling of kinases, ubiquitin ligases, and methyltransferases for target discovery and validation
- Small molecule profiling—profiling of radiolabeled, biotinylated, or fluorescently labeled small molecules for target identification or selectivity
- Protein-protein interaction profiling—Protein-protein interactions for mapping biochemical pathways
- Antibody specificity profiling—antibody specificity profiling for research and therapeutic antibody development
|Figure 2. Range of ProtoArray® applications.| A. Biomarker discovery, B. Profiling enzymatic activity—Kinase substrate identification,methyltransferase substrate identification, and ubiquitin ligase profiling, C. Protein-protein interactions and antibody specificity profiling, and D. Small molecule protein profiling.
Profiling with ProtoArray® Protein Microarrays
ProtoArray® Human Protein Microarrays enable a broad range of research applications and are available both as complete optimized kits for certain applications as well as individual arrays to support a broader range of applications. Complete kits with optimized product configurations for screening protein-protein interactions and kinases against thousands of potential native protein substrates are available. Protein-protein interaction studies can be completed in as little as one day using either a V5-tagged or biotinylated protein probe with Anti-V5-Alexa Fluor® 647 Antibody or streptavidin-conjugated Alexa Fluor® 647 for detection. Screening of kinases can be completed in as little as 2 days with no need for special instrumentation—detection can be done with either a phosphorimager or conventional autoradiography film.
Using the ProtoArray® Human Protein Microarray is simple, and you get high-quality results in a fraction of the time. Regardless of your study, fluorescent applications can be completed in as little as one day. ProtoArray® microarray applications follow the same simple workflow of incubating a sample on the array, probing with a detection reagent, and then visualizing with a fluorescent scanner or phosphorimager (Figure 3).
|Figure 3.| Simple, robust assay for discovering novel protein-protein interactions in as little as one day.
For Research Use Only. Not for use in diagnostic procedures.