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Phospho-ETS2 pThr72 Antibody (44-1105G) in ChIP
Chromatin Immunoprecipitation (ChIP) was performed using Anti-Phospho-ETS2 pThr72 rabbit polyclonal antibody (Product ## 441105G, 5 ug) on sheared chromatin from 2 million Jurkat cells using the "MAGnify ChIP system" kit (Product ## 49-2024). Normal Rabbit IgG (1 ug) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product ## 4351106) with optimized PCR primer pairs for the promoters of the active Rho-GDIbeta, MCL1 region used as positive control target gene, and the promoter region of GAPDH region, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
