Cell cycle analysis with FxCycle Violet Ready Flow Reagent and Click-iT EdU

Jurkats cells, a human T cell leukemia cell line, were pulsed with 10 µM EdU for 2 hours prior to detection with Alexa Fluor™ 647 azide. Cells were subsequently stained by adding 2 drops of FxCycle™ Violet Ready Flow™ Reagent and incubated for 30 minutes at 25°C. Data was acquired on an Attune™ NxT Flow Cytometer using a 405 laser and 440/50 nm emission filter. Analysis of the population indicates the following distribution: apoptotic sub-G1 cells were 3.4%; G0/G1, 49.1%; S: 33.0%; and G2m 14.0%.

Jurkats cells, a human T cell leukemia cell line, were pulsed with 10 µM EdU for 2 hours prior to detection with Alexa Fluor™ 647 azide. Cells were subsequently stained by adding 2 drops of FxCycle™ Violet Ready Flow™ Reagent and incubated for 30 minutes at 25°C. Data was acquired on an Attune™ NxT Flow Cytometer using a 405 laser and 440/50 nm emission filter. Analysis of the population indicates the following distribution: apoptotic sub-G1 cells were 3.4%; G0/G1, 49.1%; S: 33.0%; and G2m 14.0%.

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