Undifferentiated embryonic stem cell growth

Murine D3 cells were cultured at low density in D-MEM or KnockOut™ D-MEM supplemented with ES Cell Qualified FBS or KnockOut™ SR, traditional supplements. No LIF (Leukocyte Inhibitory Factor) was used. After 7 days, colonies were fixed and stained for alkaline phosphatase, a marker for undifferentiated ES cells. Undifferentiated colonies were scored based on morphology and staining characteristics.

Murine D3 cells were cultured at low density in D-MEM or KnockOut™ D-MEM supplemented with ES Cell Qualified FBS or KnockOut™ SR, traditional supplements. No LIF (Leukocyte Inhibitory Factor) was used. After 7 days, colonies were fixed and stained for alkaline phosphatase, a marker for undifferentiated ES cells. Undifferentiated colonies were scored based on morphology and staining characteristics.

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