Mass spectrometry (MS) workflows are poised to overcome limitations in our ability to identify and quantify proteins in single cells. In this webinar, Dr. Ryan Kelly will describe: nanoPOTS, a new sample processing platform to maximize sample recovery; miniaturization of LC separations; and latest-generation of MS instrumentation to provide the required sensitivity and throughput for effective analysis of such trace samples.
Combined, these advances increase the sensitivity and throughput of single-cell MS by orders of magnitude making it possible to routinely quantify hundreds to >1000 proteins and proteoforms from an individual cell, transforming biomedical research and the understanding of biological systems on a new level.
You will learn about:
- How to optimize and minimize sample loss in single cell sample preparation for MS analysis
- Best practices for single cell analysis with LC-MS
- Using isobaric multiplexing strategies such as TMT for maximum throughput and sensitivity
- Optimizing mass spec system for sensitivity using FAIMS technology