Fluorescent dyes successfully tested with the Image-iT FX signal enhancer—Table 7.4

Dyes * with potentially strong background fluorescence that is reduced with the Image-iT FX Signal Enhancer †
Fluorescein Oregon Green 488 Oregon Green 514 Tetramethylrhodamine Texas Red Cascade Yellow Dy 565 Dy 630 Atto 590	Atto 610 Cascade Blue Alexa Fluor 405 Alexa Fluor 430 Alexa Fluor 488 Alexa Fluor 500 Alexa Fluor 514 Alexa Fluor 555 Alexa Fluor 568	Alexa Fluor 594 Alexa Fluor 610 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750
*Dyes with potentially weak background fluorescence that is reduced with the Image-iT FX Signal Enhancer †**
Cy5 Dy 635 Marina Blue	Alexa Fluor 532 Alexa Fluor 546	Allophycocyanin R-phycoerythrin
*Background-free fluorescent dyes that are not affected by the Image-iT FX Signal Enhancer ‡**
Alexa Fluor 350 IRTM 790 Cy3	Pacific Blue Rhodamine B Rhodamine Red-X	Texas Red-X Dy 550 Dy 610
* All dyes were conjugated to streptavidin and tested at 10 µg/mL. † Background staining was blocked by the Image-iT FX signal enhancer. ‡ Staining was unaffected by the Image-iT FX signal enhancer.

For Research Use Only. Not for use in diagnostic procedures.

Fluorescent Dyes Successfully Tested with the Image-iT FX Signal Enhancer—Table 7.4