Live-cell imaging of cytoskeletal and histone dynamics during mitosis using CellLight™ reagents.

U-2 OS cells were transduced with CellLight™ Histone 2B-RFP (Cat. No. C10595) and CellLight™ MAP4-GFP (Cat. No. C10598). The next day, cells were imaged in McCoys media (12330) and a 40X objective, with images were collected every 5 minutes for 7 hours and 35 minutes. Imaging was performed on live cells using a DeltaVision® Core microscope and standard DAPI/FITC/TRITC filter sets.

U-2 OS cells were transduced with CellLight™ Histone 2B-RFP (Cat. No. C10595) and CellLight™ MAP4-GFP (Cat. No. C10598). The next day, cells were imaged in McCoys media (12330) and a 40X objective, with images were collected every 5 minutes for 7 hours and 35 minutes. Imaging was performed on live cells using a DeltaVision® Core microscope and standard DAPI/FITC/TRITC filter sets.

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