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Beta-glucuronidase (GUS) protein expression in Synechococcus elongatus
The gene for beta-glucuronidase (GUS) was cloned into vector pSyn_6 and transformed into Synechococcus elongatus (negative for GUS activity) following manufacturer's recommended protocols. GUS expression was measured by Western blot analysis and showed GUS protein represented >20% of total soluble protein. [A] SDS-PAGE gel with SimplyBlue™ staining. [B] Western blot using anti-V5 FITC antibody. TSP: total soluble protein. PUR: protein purified with Dynabeads® His-TAG Isolation & Pulldown beads.
![The gene for beta-glucuronidase (GUS) was cloned into vector pSyn_6 and transformed into Synechococcus elongatus (negative for GUS activity) following manufacturer's recommended protocols. GUS expression was measured by Western blot analysis and showed GUS protein represented >20% of total soluble protein. [A] SDS-PAGE gel with SimplyBlue™ staining. [B] Western blot using anti-V5 FITC antibody. TSP: total soluble protein. PUR: protein purified with Dynabeads® His-TAG Isolation & Pulldown beads.](http://tools.thermofisher.com/content/sfs/gallery/low/A24243.2.jpg)
