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siRNA Silencing of GAPDH Gene Expression
siRNA to human GAPDH and a scrambled control were fluorescently labeled with Cy3 using Ambion's Silencer™ siRNA Labeling Kit, transfected into HeLa S3 cells, and analyzed by fluorescence microscopy with an anti-GAPDH antibody. Red: Cy3 labeled siRNA; Blue: DAPI stained nuclei; Green: fluorescein labeled antibody to GAPDH. A. siRNA silencing of GAPDH expression in HeLa S3 cells. B. Scrambled control siRNA has no effect on GAPDH protein levels.
