Vitamin D Testing Kit: Cascadion 25-Hydroxy Vitamin D Assay

Thermo Fisher Scientific products are distributed globally so uses, applications, and availability of product in each country depend on local regulatory marketing authorization status.

A highly specific vitamin D assay kit using the gold standard reference method

Vitamin D testing is commonly used to classify Vitamin D insufficiency. Vitamin D testing often refers to quantitating 25-hydroxyvitamin D, also known as 25-OH vitamin D. Although the active form of Vitamin D is 1,25-dihydroxyvitamin D, most vitamin D is found in its storage forms, 25-OH vitamin D2 and D3, at levels up to 1,000-fold higher and with a longer half-life than the active form.1

25-OH Vitamin D assay kits are subject to interference from many sources, from Vitamin D metabolites, to common dietary supplements.2 The Centers for Disease Control and Prevention (CDC) has established the liquid chromatography–tandem mass spectroscopy (LC-MS/MS) method as the reference method for 25-OH Vitamin D testing. This method eliminates interferences and measures only the relevant fractions, ergocalciferol (D2) and cholecalciferol (D3).3,4 The Cascadion SM 25-Hydroxy Vitamin D assay uses gold standard mass spectrometry for 25-OH Vitamin D testing.

Learn more about Vitamin D test kits and methods

Benefits of the Cascadion Mass Spec Vitamin D

  • Utilizes mass spectrometry-based reference method technology
  • Complete measurement of Vitamin D2 and D3 with Total 25-OH Vitamin D reported
  • 30 day onboard internal standard stability
  • NIST-traceable controls and calibrators with a 30 day calibration stability
  • No manual preparation with ready to use internal standard, calibrators, and controls 

Cascadion Vitamin D Assay Kit Measurement Range

  1. Medicine, I. of. Dietary Reference Intakes for Calcium and Vitamin D. (The National Academies Press, 2011)
  2. Lee, J. H., Choi, J. H., Kweon, O. J., & Park, A. J. (2015). Discrepancy between Vitamin D Total Immunoassays due to Various Cross-reactivities. Journal of bone metabolism22(3), 107–112.
  3. Vesper H, Botelho J, Vitamin D Standardization Certification Program, CDC publication March 2014,
  4. Analytical Note for 25-Hydroxyvitamin D Data Analysis using NHANES III (1988-1994), NHANES 2001-2006, and NHANES 2007-2010 (October 2015), National Health and Nutrition Examination Survey, National Center for Health Statistics, CDC.