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PAX4 Antibody (PA1-108) in WB
Western blot analysis of Pax4 was performed by loading the indicated amounts of recombinant Pax3 (negative control) or Pax4 proteins, and 10ul of PageRuler Prestained Protein Ladder (Product ## 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product ## 62288), and blocked with 5% milk in TBST for at least 1 hour at room temperature. Pax4 was detected at ~38kD using a Pax4 polyclonal antibody (Product ## PA1-108) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugate goat anti-rabbit IgG secondary antibody (Product ## 31460) at a dilution of 1:40,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal West Pico (Product ## 34080). NOTE: The presence of Pax3 recombinant protein was determined by probing a duplicate blot with a Pax3 polyclonal antibody (Product ## PA1-107), and the corresponding image is on the webpage for Product ## PA1-107.
