Qnuclear™ Deep Red Stain

Qnuclear™ Deep Red stain is a bright and photostable nuclear counterstain designed for use with cells labeled with Qdot nanocrystals and mounted in Qmount™ Qdot mounting media. It can be visualized with standard fluorescence microscopy filter sets.

 

How Qnuclear™ Deep Red Stain Works

With excitation and emission maxima of 640 and 663 nm, respectively, Qnuclear™ Deep Red stain can be visualized with standard fluorescence microscopy filter sets (Figure 1) and is completely compatible with Qdot 525, 565, 585, 605, and 625 nanocrystals. This counterstain can also be used with Qdot 655 nanocrystals and its conjugates, though care must be taken to use appropriate excitation and filter sets given the fluorescence emission overlap. The fluorescent signal from Qnuclear™ Deep Red stain is well-maintained when mounted in the recommended Qmount™ Qdot mounting media (Figure 2). Qnuclear™ Deep Red stain is provided as a convenient, concentrated dimethyl sulfoxide (DMSO) solution.


Qnuclear™ Deep Red Stain absorption and emmission

Figure 1—Absorption and fluorescence emission spectra for Qnuclear™ Deep Red stain.


Qnuclear™ Deep Red Stain comparison

Figure 2—Comparison of human carcinoma (HeLa) cells labeled with Qnuclear™ Deep Red stain and mounted in Qmount™ Qdot mounting media. Samples were imaged 1 day, 13 days, and 8 months after mounting. Qmount™ Qdot mounting media provided excellent signal preservation both initially and when stored over time, as measured by fluorescence intensity in the nuclear regions.

Qmount™ Qdot Mounting Media

Qmount™ Qdot Mounting Media

Qmount™ Qdot mounting media preserves the fluorescence signal of Qdot nanocrystals with little to no loss of the signal’s intensity both initially and over the course of several months.