Welcome to the Thermo Scientific resource library. This page contains valuable links and supporting resources for standard molecular biology techniques, as well as specialty applications. It also provides easy access to a large variety of Thermo Scientific webinars, videos, articles, and more.

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Articles highlighting key advances, technical tips, and new product capabilities

Workflow solutions for molecular biology

Links to help you find the right Thermo Scientific product for your specific project

Specific molecular biology applications

Information covering techniques including qPCR, mutagenesis, sequencing, epigenetics, in vitro transcription, genotyping, labeling, and detection

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Webinars

Educational presentations focused on a variety of scientific techniques and solutions

Web tools

Online tools for analyzing primers, setting up reactions, and performing biochemical conversions and calculations

Thermo Scientific selection guides

PCR enzymes and master mixes
Reverse transcriptases
DNA ladders
PCR plastics

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在线工具

引物分析、反应体系建立、进行生化转换及计算的在线工具.

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Videos

Reverse transcription

Thermo Scientific Maxima First Strand cDNA Synthesis Kits with double-stranded DNase combine genomic DNA elimination and cDNA synthesis into a simple, one–tube workflow that can be completed in as few as 15 minutes.


PCR

In this video we will show you how to set up a PCR reaction using Thermo Scientific Phusion High-Fidelity DNA Polymerase.

There are two protocols with the Thermo Scientific Direct PCR Kits: the direct protocol and the dilution and storage protocol. In this video, we will briefly demonstrate how they work.

How to video to demonstrate how to perform colony PCR as part of a cloning workflow using Thermo Scientific DreamTaq DNA Polymerase.

In this video, we will demonstrate how to amplify DNA directly from an unpurified sample using Thermo Scientific Direct PCR Kits. The master mix format enables robust PCR that can be completed in less than 30 minutes.


PCR plastics

This video explains different formats of PCR/qPCR plates and how to choose an optimal fit for your instrument.

This video demonstrates how to properly seal a PCR/qPCR plate with an adhesive film and cap strips.

This video reviews plate features important for robotic liquid handlers in high-throughput PCR/qPCR applications.

Thermo Scientific VersiPlate and VersiCap Mats are flexible consumables for PCR and qPCR making set-up and handling simple and easy.


Nucleic acid electrophoresis

Forget the Powder! Watch this how to video about new Thermo Scientific TopVision Agarose Tablets, which eliminate the need to weight out messy powdered agarose.

When running agarose gel electrophoresis, problems can arise from the following usage areas: improper gel preparation, overloading the gel, and inappropriate electrophoresis parameters. In this video, we will take a look at them one by one.


Molecular cloning

How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.

The Thermo Scientific CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any other blunt or sticky-end DNA fragment can be cloned.

Our Center of Excellence for Molecular Biology located in Vilnius, Lithuania is the world leader in the field of enzyme research and development, where over 30% of all known restriction enzymes were discovered/characterized.

Double digest your DNA in only 5-15 minutes with one buffer and load directly onto your gel with 176 Thermo Scientific FastDigest Enzymes.


Restriction and modifying enzymes

PCR相关资源

PCR相关文章

PCR在线工具

PCR在线讲座

在该在线讲座中我们将讨论DNA聚合酶技术的改进。我们将探讨针对不同的PCR应用如何选择最佳的DNA聚合酶,以及如何合理地组织PCR实验流程.

直接PCR:无需核酸纯化即可进行扩增

该讲座回顾了直接PCR方法的基本原理,介绍了最常见的实验应用,同时提供了对于不同样品类型应如何进行直接PCR的指南.

优化您的PCR

在该讲座中,我们将回顾PCR的基础知识,并对反应体系建立、反应优化、以及数据分析的最佳范例进行讨论.

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qPCR相关资源

qPCR在线工具

qPCR在线讲座

在该在线讲座中我们回顾了qPCR流程中最常见的变量及错误的来源,并讨论了如何避免这些问题.

主题包括:qPCR原理及相关术语,检测化学反应,扩增图及Cq值,绝对定量vs.相对定量,以及检测设计要素等.

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限制性内切酶相关资源

限制性内切酶相关文章

限制性内切酶在线工具

限制性内切酶在线讲座

在该在线讲座中我们将回顾与限制性内切酶消化相关的最常见问题,并给出创新性解决方案。我们还将为您介绍避免发生不完全酶切的要诀和技巧,并解释出现的非预期的剪切模式及如何在克隆过程中以最佳方式使用RE.

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反转录相关资源

反转录相关文章

反转录在线工具

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核酸电泳及核酸纯化相关资源

核酸电泳及纯化相关文章

核酸电泳及核酸纯化在线工具

核酸电泳及核酸纯化在线讲座

在该在线讲座中我们将对植物研究课题进行概要性的介绍。我们将重点关注加速植物样品处理的要诀和技巧,另外还将讨论针对核酸纯化和PCR的植物特殊解决方案.

在该讲座中我们将讨论核酸纯化流程的主要步骤,包含从样品收集到核酸保存等;并分享如何优化得率以及如何精确地对提取核酸的纯度和浓度进行评估的技巧和建议.

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分子克隆相关资源

分子克隆相关文章

分子克隆在线工具

分子克隆在线讲座

在该在线讲座中我们将对不同的DNA末端修饰方法、如何建立最优化的连接反应、如何评估并提高连接效率等等进行回顾。另外,我们还将讨论传统的T4 DNA连接酶克隆方法的替代方法,例如PCR克隆和LIC等.

该在线讲座涵盖了起始材料纯化、插入片段及载体制备、DNA末端修饰、连接、转移、以及阳性克隆分析筛选等多方面内容。建议在实验流程中的每一步都增加必需的对照.

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测序相关资源

测序在线讲座

在该讲座中我们将对常规的NGS文库制备流程的每个步骤进行讨论,同时引入一种全新的转座子方法。另外还会介绍最佳范例以及重要的质量控制检查点等.

测序相关文章

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体外转录相关资料

体外转录相关文章

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