Maxima H Minus RTs are engineered with molecular evolution techniques selecting for mutations that confer dramatically improved thermostability, processivity and robust activity rates compared with wild-type M-MuLV enzymes. These features support higher overall cDNA yields with a variety of templates and improved synthesis from templates with complex secondary structures.
Maxima H Minus RT also shows greater efficiency with synthesis of full length cDNA templates. It lacks RNase H activity that normally degrades RNA from RNA-DNA duplexes as the cDNA is being synthesized; which helps to prevent premature degradation of RNA. In RT-qPCR applications, Maxima H Minus RT also enables high efficiency synthesis over a wide range of input template amounts providing sensitive and accurate quantification of cDNA.
Maxima H Minus Reverse Transcriptase outperforms other reverse transcriptases, producing high yields of full-length cDNA over a wide temperature range (Figure 1). Its tolerance of high reaction temperatures allows for efficient transcription of RNA regions with extensive secondary structure and helps to improve primer specificity increasing overall yields.
Designed with proprietary mutations for enhanced processivity, Maxima H Minus RT has 50x increase in processivity compared with wild type MMuLV RT enzymes. This RT is capable of synthesizing full-length cDNA from a wide range of RNA templates (Figure 2).
Efficient cDNA synthesis
Maxima H Minus RT is capable of efficient cDNA synthesis from a wide range of template amounts, outperforming other suppliers’ RTs with higher yields and better linearity in cDNA synthesis making it an ideal choice for RT-qPCR experiments (Figure 3). The premixed solutions in the Thermo Scientific Maxima First Strand cDNA Synthesis Kits help further improve reproducibility and save time during reaction setup.