Platinum Taq DNA Polymerase shows more coverage for more amplicons.

Products amplified with Platinum Taq DNA Polymerase (A); the same products amplified with Sigma JumpStart™ DNA Polymerase (B). PCR reactions were run using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate.

Data produced with Platinum Taq DNA Polymerase (A); the same amplicons amplified using the Qiagen HotStarTaq DNA Polymerase (B). PCR reactions were performed using 1 ng of template DNA and 1.25 units of enzyme in each 50 µL reaction. Annealing was uniform across the selected targets. Annealing temperatures were uniform across the amplicon panel. Amplicons ranged from 300 to 1,400 bp in length, with an average length of 553 bp. Each reaction was performed in duplicate.