Q: What is the AmpFℓSTR Identifiler Direct PCR Amplification Kit?
A: The Identifiler Direct kit is a short tandem repeat (STR) kit developed to directly amplify the 16 loci included in the Identifiler kit from single source samples spotted on FTA cards (e.g. offender DNA database samples, paternity samples, casework reference samples); no DNA extraction or purification is required.

Q: When will the Identifiler Direct kit be available?
A: The kit is expected to be available in the fall of 2009. A trial-size kit will be available at this time, but only as an introductory offer. Trial-size kits will contain 200 reactions, with a maximum of 1 kit orderable per laboratory. The trial-size kit will be distributed with a customer letter that describes the kit, and includes several recommended experiments to help customers evaluate the kit.

Q: Is the Identifiler Direct kit sold in different package sizes?
A: The Identifiler Direct kit is available in one package size of 1000 reactions. Trial size kits with 200 reactions will be available for a limited time when the kit is launched (see above).

Q: Were the primer sequences changed from the primer sequences used in the Identifiler kit?
A: No, the Identifiler Direct kit uses the same primer sequences as the Identifiler kit.

Q: What are the automation requirements for the Identifiler Direct kit?
A: By eliminating DNA extraction and purification, the Identifiler Direct kit facilitates high-throughput processing with a simple, easy-to-automate protocol that requires a less sophisticated and less expensive robotic platform. Identifiler Direct PCR reactions can be set-up using any basic liquid handling system capable of precise and reliable pipetting of low volumes of liquids (i.e. 25µl). In addition, the Identifiler Direct kit will be validated by Applied Biosystems for use with the HID EVOlution™ System.

Q: On which CE instrument platforms is the Identifiler Direct kit supported?
A: The Identifiler Direct kit is designed for use in medium-high throughput environments, and therefore is being internally validated on the 3130xl and 3730 platforms only. The Identifiler Direct kit can also be run on the 3100 and 3130 platforms, and some customer test site laboratories plan on validating the Identifiler Direct kit on these platforms. However, because of the medium-high throughput focus of the Identifiler Direct kit, the 310 platform will not be supported.

Q: Will the Identifiler Direct kit work with any punch size, or with different PCR reaction volumes?
A: The Identifiler Direct Kit is optimized specifically for 1.2mm discs in a 25µl PCR reaction volume. The kit is designed to overcome the inhibition present when processing unpurified samples in a 25µl PCR reaction volume, and increasing the disc size or reducing the PCR reaction volume can cause the quality of the results to deteriorate.

Q: Will the Identifiler Direct kit work with single source samples that are not spotted on FTA cards?
A: The chemistry has been optimized to work on buccal and blood samples spotted on FTA cards. In order to use a blood or buccal sample that is not spotted on an FTA card, a separate lysis step is required.