Improving upon innovations in buffer formulation made during the development of earlier AmpFℓSTR Kits such as the MiniFiler™ Kit, the Identifiler Plus Kit has been designed to help overcome high levels of PCR inhibition, and promote a more robust and efficient amplification. The new formulation helps deliver results from samples which could not be analyzed using the previous generation Identifiler Kit.
Figure 1. A comparison of the performance of the Identifiler Plus Kit with the Identifiler Kit on an inhibited casework sample. The Identifiler Kit is unable to amplify a blood sample from a leather glove. The Identifiler Plus kit delivers a full profile as a result of its enhanced formulation. 1ng input DNA and 28 cycles of amplification were used for both kits.
Figure 2. A study demonstrating the performance of the Identifiler Plus Kit in the presence of increasing concentrations of common PCR inhibitors hematin and humic acid. Even at the highest concentrations, the Identifiler Plus Kit shows the ability to recover full profiles. 1ng input DNA and 28 cycles of amplification were used for all replicates.