Q: Which loci are included in the MiniFiler™ Kit?
A: The MiniFiler™ Kit is a 9-plex which includes: D13S317, D7S820, Amelogenin, D2S1338, D21S11, D16S539, D18S51, CSF1PO, and FGA.
Q: How do I integrate the MiniFiler™ Kit into my current sample workflow?
A: The MiniFiler™ Kit is an adjunct kit to the Applied Biosystems autosomal STR kits that are currently being used for forensic casework. Loci that drop out in DNA samples, which produce partial profiles using currently available STR kit technology, may be recovered using the MiniFiler™ Kit. The MiniFiler™ Kit has been optimized to produce results from degraded and/or inhibited samples.
Q: Can the profiles produced with the MiniFiler™ Kit be uploaded into CODIS?
A: At the present time the MiniFiler™ Kit is still in development and therefore has not been accepted by NDIS. When the kit is available in the final manufactured format the appropriate experiments will be conducted and submitted to NDIS for approval.
Q: Will the MiniFiler™ Kit gain acceptance by ENFSI and GEDNAP?
A: An ENFSI / GEDNAP ring trial is in the planning phase. When the MiniFiler™ Kit is available in the final manufactured format a formal ring trial will proceed. A journal article will be authored and published in a peer-reviewed journal once complete.
Q: I am interested in bringing the MiniFiler™ Kit into my lab when available. What do I need to prepare for integration?
A: The following:
- Ability to run 5-dye applications – The MiniFiler™ Kit uses the same size standard, matrix standard and capillary electrophoresis modules as Identifiler, Yfiler and SEfiler™ Kits.
- GeneMapper ID Software v3.2 – Data from the MiniFiler™ Kit can not be analyzed with GeneScan/Genotyper Software.
- New panel and bin set for GeneMapperID software.
Q: When will the MiniFiler™ Kit be available for purchase?
A: The kits will be commercially available early in calendar year 2007.
Q: What types of samples can I analyze using the MiniFiler™ Kit?
A: Any casework, missing person or disaster victim sample, which is degraded, inhibited or otherwise compromised, producing an incomplete profile using traditional STR technology. This includes: bone, hair, blood, saliva, swabs, tissue, "touch" samples, etc.