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BioTek’s Synergy™ H4 and Synergy™ 2 multi-mode microplate readers have received the LanthaScreen Certified Plus designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before Using LanthaScreen Terbium and Europium Assays

Test Your Plate Reader Set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note

Download Europium assay application note

Synergy™ H4 Hybrid Multi-Mode Microplate Reader

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note View View View View View
Poster - View View View View
Excitation Filter (18 mm)  340/30 400/30 340/30 530/25  400/30 
Emission 1 Filter (18 mm)  620/10 460/40 495/10 590/35 460/40 
Emission 2 Filter (18 mm)  665/8 528/20 520/25 590/35  528/20 
Dichroic Mirror  400 NA 400  570 435 

 

Synergy™ 2 Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note View View View View View
Poster - View View View View
Excitation Filter (18 mm) 340/30 400/30 340/30 530/25  400/30 
Emission 1 Filter (18 mm) 620/10 460/40 495/10 590/35 460/40 
Emission 2 Filter (18 mm) 665/8 528/20 520/25 590/35  528/20 
Dichroic Mirror 400 NA 400 570 435

Synergy™ HT Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not compatible

Reader not tested

Reader not compatible

Reader not compatible

Reader not tested

Application Note - View - - View
Poster - - - - -
Excitation Filter (18 mm) - 400/30 - - 400/30 
Emission 1 Filter (18 mm) - 460/40 - - 460/40 
Emission 2 Filter (18 mm) - 528/20 - - 528/20 

FLx800™ Fluorescence Microplate Reader

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not compatible

Reader not compatible

Reader not compatible

Application Note - - - - -
Poster - View - - View
Excitation Filter (18 mm) - 400/30 - - 400/30 
Emission 1 Filter (18 mm) - 460/40 - - 460/40 
Emission 2 Filter (18 mm) - 528/20 - - 528/20 



* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm). Contact BioTek Instruments for information on ordering filters and dichroic mirrors suitable with the microplate readers listed above. 

Synergy™ and FLx800™ are trademarks of BioTek Instruments.