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The Analyst, FlexStation and SpectraMax M5/M5e from Molecular Devices have received the LanthaScreen Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note

Download Europium assay application note

SpectraMax Paradigm Microplate Detection Platform

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide
Application Note
Poster - -
Excitation Filter   406/15   532/25  
Emission 1 Filter   465/35   595/35   
Emission 2 Filter   535/25   595/35   
Dichroic Mirror   508      
Optic Module   GeneBLAzer   FP rhodamine   

 

SpectraMax M5/M5e Microplate Reader
& FlexStation 3 Benchtop Multi-Mode Microplate Reader


 
 

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not
compatible

Application Note - - - - -
Poster - - - - -
Excitation 332/12 410/12 332/12   410/12
Emission 1 620/12 458/12 488/12   458/12
Emission 2 666/12 522/12 518/12   522/12
Dichroic Mirror 590/630 435/495 420   435/495

FilterMax™ F5 Multi-Mode Microplate Reader

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide Reader not compatible Reader not compatible Reader not compatible
Application Note
Poster - -
Excitation Filter   405/30      405/30
Emission 1 Filter   465/35      465/35
Emission 2 Filter   535/25      535/25
Dichroic Mirror          

 

Analyst Multimode Reader


     

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

-

-

 

-

-

Application Note - - - - -
Poster - - - - -
Excitation Filter (25 mm) 340/30 (PV00225) 405/20 340/30 (PV00225) 530/25 405/20
Emission 1 Filter (25 mm) 620/7.5 460/40 495/10 (PV00325) 580/10 460/40
Emission 2 Filter (25 mm) 665/7.5 530/25 520/25 (PV00325) 580/10 530/25
Dichroic Mirror 380 or
400
425 or
50/50
380 or
400
561 425 or
50/50

 

* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above.

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information.

Analyst, SpectraMax and FlexStation are registered trademarks of Molecular Devices.