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Tecan's Infinite M1000/M1000 PRO and Infinite F500 have received the LanthaScreen Certified Plus designation and Tecan’s Infinite F200/200 PRO have received the LanthaScreen Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.

Before using LanthaScreen Terbium and Europium assays

Test your plate reader set-up

These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.

Download Terbium assay application note

Download Europium assay application note

Infinite M1000/M1000 PRO

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - - - View -
Poster - - - - -
Excitation 317/20 409/12 332/20 530/5 405/12
Emission 1 620/12 460/12 490/10 585/20 445/12 
Emission 2 665/12 530/12 520/20 585/20  520/12 

 

Infinite F500

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - -   View -
Poster - - - - -
Excitation Filter (12.5 mm) 340/30
(PV002125)
415/20 340/30
(PV002125)
535/25 415/20
Emission 1 Filter (12.5 mm) 615/10 460/20 495/10
(PV003125)
590/20 460/20 
Emission 2 Filter (12.5 mm) 665/10 535/25 520/25
(PV003125)
590/20  535/25
Dichroic Mirror 510 NA 50% or 510 560 50%

 

Infinite F200/200 PRO

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Reader not tested

Application Note - - - - -
Poster - - - - -
Excitation Filter (12.5 mm) 340/30
(PV002125)
409/20 340/30
(PV002125)
NA 415/20
Emission 1 Filter (12.5 mm) 615/10 460/20 495/10
(PV003125)
NA 460/20 
Emission 2 Filter (12.5 mm) 665/10 525/25 520/25
(PV003125)
NA 535/25
Dichroic Mirror 510 NA 50% or 510 NA 50%

 

Safire2

 

Instrument Configuration:

Assay Technology  Adapta &
LanthaScreen
Europium
GeneBLAzer LanthaScreen
Terbium
Predictor™ Z'-LYTE
Modality TR-FRET FRET (bottom) TR-FRET FP FRET
Setup Guide

Application Note - - - - -
Poster - - View - -
Excitation 317/20  409/12 332/20 530/20 405/12 
Emission 1 620/12 460/12 485/20 585/20 445/12 
Emission 2 665/12 530/12 515/20 585/20 520/12 



* Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Tecan for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above

** Some compatible filter sets are available for purchase from Life Technologies.  Click on selected filter sets for more information

Infinite is a registered trademark and Safire2™ is a trademark of Tecan Group Ltd.