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We offer a broad range of Alexa Fluor fluorescent streptavidin conjugates (Table 1 and Table 3) and avidin conjugates (Table 2). These dyes represent a major breakthrough in the development of fluorescent labeling reagents and, without exception, produce the best and brightest conjugates we have ever tested. Benefits of the Alexa Fluor dyes and their conjugates include exceptionally bright conjugatesexcellent photostability, and a broad range of emission colors. These characteristics make Alexa Fluor dyes the best choice for most imaging applications.

Table 1. Alexa Fluor Fluorescent Streptavidin Conjugates

Streptavidin Conjugates   Ex (nm)* Em (nm)*  Cat . No.  (Unit Size)
Alexa Fluor 350 Streptavidin 346 442 S11249 (1 mg)
Alexa Fluor 405 Streptavidin 402 421 S32351  (1 mg)
Alexa Fluor 430 Streptavidin 434  539 S11237  (1 mg)
Alexa Fluor 488 Streptavidin 495 519

S11223 (1 mg)

S32354 (0.5 mL)

Alexa Fluor 500 Streptavidin 503 525 S32352  (1 mg)
Alexa Fluor 514 Streptavidin 518 540 S32353  (1 mg)
Alexa Fluor 532 Streptavidin 530 554 S11224  (1 mg)
Alexa Fluor 555 Streptavidin 555 565

S21381 (1 mg)

S32355  (0.5 mL)

Alexa Fluor 546 Streptavidin 556 573 S11225 (1 mg)
Alexa Fluor 568 Streptavidin 578 603 S11226  (1 mg)
Alexa Fluor 594 Streptavidin 590 617

S11227 (1 mg)

S32356  (0.5 mL)

Alexa Fluor 610 Streptavidin 612 628 S32359  (1 mg)
Alexa Fluor 633 Streptavidin 632 647 S21375  (1 mg)
Alexa Fluor 635 Streptavidin 633 647 S32364 (1 mg)
Alexa Fluor 647 Streptavidin 650 668

S21374 (1 mg)

S32357 (0.5 mL)

Alexa Fluor 660 Streptavidin 663 690 S21377 (1 mg)
Alexa Fluor 680 Streptavidin 679 702

S21378 (1 mg)

S32358 (0.5 mL)

Alexa Fluor 700 Streptavidin 702 723 S21383 (1 mg)
Alexa Fluor 750 Streptavidin 749 775 S21384   (1 mg)
*Approximate fluorescence excitation (Ex) an d emission (Em) maxima for conjugates, in nm.

Alexa Fluor Streptavidin Fluorescent Conjugates in Action

microscopy-analysis-muntjac-fibroblasts

Multicolor fluorescence microscopy analysis of muntjac fibroblasts. The actin cytoskeleton was labeled with biotin phalloidin and visualized using Alexa Fluor 350 streptavidin. Mitochondria were stained with MitoTracker Red CMXRos; the nucleus was labeled with an anti-phosphohistone primary antibody and visualized using Alexa Fluor 500 goat anti–mouse IgG.

The cytoskeleton of a fixed and permeabilized bovine pulmonary artery endothelial cell.Tubulin was detected using mouse monoclonal anti–α-tubulin antibody and visualized with Alexa Fluor 647 goat anti–mouse IgG antibody (pseudocolored magenta). Endogenous biotin in mitochondria was labeled with green-fluorescent Alexa Fluor 488 streptavidin; DNA was stained with blue-fluorescent DAPI.

permeabilized-bovine-pulmonary-artery-endothelial-cell

Table 2. Alexa Fluor NeutrAvidin and Avidin Conjugates

Fluorescent  Conjugate  Ex (nm)* Em (nm)*  Cat . No.
Alexa Fluor 350  Neutravidin
346
442
A11236
Alexa Fluor 488  Avidin
495
519
A21370

*Approximate fluorescence excitation (Ex) an d emission (Em) maxima for conjugates, in nm.

Alexa Fluor–Phycobiliprotein Streptavidin Conjugates

Life Technologies™ offers fluorescent streptavidin conjugates of with tandem dyes R-PE (R-Phycoerythrin) and APC (Allophycocyanin) labeled with long-wavelength Alexa Fluor dyes. Our tandem conjugates (Table 3) comprise a donor phycobiliprotein—R-PE or APC—conjugated to longer wavelength–emitting fluorescence acceptors. By the process of fluorescence resonance energy transfer (FRET), an energy transfer cascade is established wherein most of the light absorbed by the donor R-PE or APC results in fluorescence of the acceptor dye. This process can be quite efficient, resulting in almost total transfer of energy to the acceptor dye.

Table 3. Tandem Alexa Fluor–Phycobiliprotein Streptavidin Conjugates

R-PE, B-PE, and Tandem–R-PE Conjugates
Streptavidin Conjugates   Ex (nm)* Em (nm)*  Cat . No.  (Unit Size)
Alexa Fluor 610–R-PE  Streptavidin
496, 546, 565 †
630
S20982
Alexa Fluor 647–R-PE  Streptavidin
496, 546, 565 †
668
S20992
Alexa Fluor 680–R-PE  Streptavidin
496, 546, 565 † 
702
S20985
Alexa Fluor 750–R-PE  Streptavidin
496, 546, 565 † 
771
S32363
APC and Tandem-APC Conjugates
Streptavidin Conjugates   Ex (nm)* Em (nm)*  Cat . No.  (Unit Size)
Allophycocyanin (APC)  Streptavidin
650
660
S868, S32362
Alexa Fluor 680–APC  Streptavidin
650
702
S21002
Alexa Fluor 700–APC  Streptavidin
650
723
S21005
Alexa Fluor 750–APC  Streptavidin
650
775
S21008
*Approximate fluorescence excitation (Ex) an d emission (Em) maxima for conjugates, in nm

All-in-One Kits for Fluorescence Imaging

The Image-iT FX Kits provide all of the dyes and reagents you need for optimal immunofluorescence imaging of fixed cells and tissue sections using Alexa Fluor fluorescent streptavidin conjugates. Each kit includes:
A pack of two CultureWell (Grace Bio-Labs) chambered cover glasses is also included for convenient processing. Each Image-iT FX Kit provides sufficient materials to perform 50–100 assays.