Zinc concentrations in the 1–100 nM range are routinely measured using fluorescent indicators nominally designed for Ca2+ detection, but higher Zn2+ concentrations are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists.
We’ve developed a number of Molecular Probes ion indicators to track zinc with intense fluorescent signals and a range of wavelength options.
Indicators for Zinc
FluoZin™ dyes are unique indicators designed for detection of Zn2+ in the 0.1–100 µM range with minimal interfering Ca2+ sensitivity. They provide bright signals for assaying exocytotic release of Zn2+ from pancreatic β-cells after stimulation with glucose or potassium. Zn2+ concentrations can be measured using imaging platforms, cell-based microplate assays, or flow cytometry.
Human vascular smooth muscle cell loaded with FluoZin™ indicator and stained with Hoechst 3342.
Used for spatial and temporal resolution of Zn2+ concentrations.
|Readout||Responds to Zn2+ concentrations in synaptic vesicles with increasing fluorescence||Responds to saturating levels of Zn2+ with 50-fold increase in fluorescence emission|
|Range||0–100 µM range with minimal interfering Ca2+ sensitivity||Low nM to µM range with minimal interfering Ca2+ sensitivity|
|Standard filter set||FITC|
|Cell permeant or impermeant?||Impermeant||Permeant||Impermeant||Permeant|
|Usage notes||Load by microinjection, patch pipette, or pinocytic loading agent||Load in cell media||Load by microinjection, patch pipette, or pinocytic loading agent||Load in cell media|
|Format||500 µg||50 µg||500 µg||100 µg|
|Molecular Probes Handbook