Detecting low abundance antigens with even the best conventional dye conjugates can be a challenge, as photobleaching can make it difficult to effectively observe and record staining. The exceptional photostability of Qdot Nanocrystal conjugates can provide substantial benefits in the detection of low abundance targets. Due to their narrow and symmetric emission spectra, Qdot nanocrystals are also ideal for multicolor, multiplexed fluorescence detection using a single excitation source such as the 405 nm laser.

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Qdot Streptavidin Conjugates

Qdot streptavidin conjugates combine the unsurpassed photostability of Qdot nanocrystals with the highly specific binding properties of streptavidin. These powerful fluorescence detection reagents offer unique performance advantages in a wide variety of tissue labeling and flow cytometry experiments; they are efficiently excited using the 405 nm violet laser, and the Qdot Nanocrystal fluorescence is extremely resistant to photobleaching.

Qdot Streptavidin Conjugate contents

These products provide 200 µL of the Qdot Streptavidin conjugate and a detailed protocol outlining the use of the product in immunohistochemistry (IHC) applications using fluorescence microscopy. At conventional working concentrations, ~100 staining experiments may be performed

Qdot Strepavidin Conjugate Sampler Kit contents

Each Qdot Streptavidin Sampler Kit provides 50 µL of each of six colors of Qdot streptavidin conjugate (525, 565, 585, 605, 655, and 705 nm emission) and a detailed protocol describing the use of the product in immunohistochemistry (IHC) applications using fluorescence microscopy. At conventional working concentrations, ~20 staining experiments with each color may be performed.

Qdot Secondary Antibody Conjugates

Qdot secondary antibody conjugates are ideal immunoreagents for use in fluorescence microscopy, microplate-based assays, flow cytometry, and many other applications. These conjugates are made with the F(ab')2 fragment of affinity-purified goat antibodies, cross-adsorbed against the serum proteins of numerous species. Qdot secondary antibody conjugates can be used in standard physiological buffers with BSA present from pH 4–9 in a variety of high-salt conditions, to achieve high-quality staining with incredibly low background.

Qdot Secondary Antibody Conjugate contents

Each kit is supplied with 200 µL of the Qdot secondary antibody conjugate and a protocol describing the use of the product in immunohistochemistry applications using fluorescence microscopy. At conventional working concentrations, approximately 100 staining experiments may be performed.

Qdot Conjugated Primary Antibodies and WGA

Fusion tags such as glutathione-s-transferase (GST) can greatly simplify purification and detection of proteins that are not yet well-characterized or do not elicit a strong immune response to produce antibodies. Our Qdot 655 anti-glutathione-s-transferase antibody conjugate can be used to detect GST-tagged proteins in western blotting, fluorescence microscopy or microtiter plate based applications.

In addition to being widely used as a fluorochrome, fluorescein is an excellent hapten that can be recognized by secondary detection reagents in a variety of applications. Many primary or secondary detection reagents, such as nucleic acid probes and antibodies, can be effectively linked to fluorescein and subsequently detected by Qdot anti-fluorescein conjugates. Anti-fluorescein conjugates also provide an alternative to a traditional biotin–avidin system. Essentially all of the methods that use biotin and avidin reagents are amenable to the fluorescein–anti- fluorescein system, which helps avoid the background caused by endogenous biotin in cells and tissues in some applications.  Also available is the  Qdot 655 rat anti-dinitrophenol conjugate for detection of the hapten, dinitrophenol (DPH).

Wheat germ agglutinin is a 36,000 molecular weight protein that binds to N-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids. There are many uses for WGA conjugates including cell surface labeling and measuring retrograde neuronal transport. Our Qdot 655 wheat germ agglutinin conjugate provides highly sensitive labeling of N-acetylglucosamine residues, with low non-specific binding.

Qdot primary conjugate product contents

Each unit is supplied with 200 µL of the Qdot conjugate and a protocol describing the use of the product in fluorescence microscopy applications.

We also have an extensive array of Qdot primary antibodies that are validated for flow cytometry.

Qdot Antibody Conjugation Kits

Qdot antibody conjugation kits allow you to conjugate your antibodies to Qdot nanocrystals. Kits are available in a wide range of colors—525, 565, 605 655, 705 or 800 nm emission—and the procedure can be completed in a few hours. The conjugation method is based on the fast and efficient coupling of thiols to maleimide groups.

Qdot antibody conjugates generated with these kits are ideally suited for applications such as immunohistochemistry, tissue staining, ELISA, Western blotting, flow cytometry, and other user-designed experiments. In addition to conjugation of antibodies, other thiol-containing molecules can be coupled to Qdot nanocrystals using this kit.

Qdot Antibody Conjugation Kits contents

The kits contain all necessary reagents and components for two conjugation reactions, each using 300 µg of an IgG antibody, as well as a detailed protocol.

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