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Imaging live animals

Novel techniques for imaging biological processes in live animals is changing how cellular activity, effects of drugs, and disease progression are monitored. We offer a range of reagents and kits with Invitrogen Molecular Probes near-infrared (NIR) fluorescent dyes and conjugates along with our Qdot probes. Areas of focus include vascular imaging and inflammation as well as optimal antibody labeling.

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Technical resources for small animal in vivo imaging

SAIVI reagents and kits used in scientific posters & presentations

Optical Imaging Probes for in vivo Tumor Biology. T.H. Steinberg, J.K. Nyhus, B. Aggeler, L. Greenfield, and L. Leong
Cambridge Healthtech Institute's Third Annual IN VIVO MOLECULAR IMAGING: Moving from Discovery to Pre-Clinical to Clinical Applications, Nov 2006

We have developed contrast agents with transient vascular imaging properties ranging from a few minutes to a few hours. We show the determination of an optimal degree of labeling (DOL; mole fluorophore/mole probe) for targeted antibodies with an NIR Alexa Fluor dye and demonstrate transient imaging and multiplexed imaging of vascularized tumors in nu/nu mice.

Products used:  Alexa Fluor 750 SAIVI Rapid Antibody Labeling Kit, Alexa Fluor 680 SAIVI Rapid Antibody Labeling KitQtracker 655 Vascular Label, and SAIVI Alexa Fluor 750 Injectable Contrast Agent, bovine serum.

Check out this poster as well:
Vascular Contrast Agents for Optical in vivo Imaging. J.K. Nyhus, T.H. Steinberg, L. Leong and L. Greenfield. SMI, 2006.

Frequently Asked Questions about Small Animal In Vivo Imaging

    Q.   What is DOL?

    A.  Degree of Labeling (DOL) describes the number of fluorophores per antibody.   For in vivo labeling experiments, the DOL is restricted to a narrow range because it has significant consequences for the biodistribution and clearance of the probe.  We have determined that the DOL range for the NIR Alexa Fluor dyes is 1.5 to 3 molecules per antibody for optimal optical in vivo imaging.

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