由于没有哪一项单独的参数能够确定细胞程序性死亡,为了获得可靠的凋亡检测结果,建议将多种方法组合使用。 我们的多参数细胞凋亡分析试剂盒旨在从单一细胞群中选择性地从活细胞和坏死细胞中区分出凋亡细胞。 该试剂盒设计用于在一次流式细胞试验中检测多项凋亡参数,有助于早期和中期凋亡反应的区分。


Multiparametric apoptosis analysis using the Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis kit with PI. Jurkat cells (T-cell leukemia, human) were treated with 10 μM camptothecin for four hours (bottom panel) or left untreated as a control (top panel). Cells were then treated with the reagents in the kit and analyzed by flow cytometry. Note that the camptothecin-treated cells (bottom panel) have a higher percentage of apoptotic cells (indicated by “A”) than the basal level of apoptosis seen in the control cells (top panel). L = live cells, D = dead cells.
 产品 Parameter 1
Parameter 2 Parameter 3 Excitation (nm)
目录号
Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor® 488 annexin V and SYTOX® Green
Membrane Asymmetry
坏死
--
488
V13240
Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit, with PI
Membrane Asymmetry
坏死
--
488
V13241
FITC Annexin V/Dead Cell Apoptosis Kit, with PI
Membrane Asymmetry
坏死
--
488
V13242
Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO®-1 and PI
Membrane Asymmetry
坏死
--
488
V13243
Chromatin Condensation Apoptosis Kit, with Hoechst 33342 and PI
Chromatin Condensation
坏死
--
UV/488
V13244
Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO®-1, and PI
Chromatin Condensation
坏死
--
UV/488
V23201
PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX® Green
Membrane Asymmetry
坏死
--
488
V35112
APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX® Green
Membrane Asymmetry
坏死
--
488/633
V35113
Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C12 resazurin, APC annexin V, and SYTOX® Green
线粒体健康
Membrane Asymmetry
坏死
488/633
V35114
Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker® Red and Alexa Fluor® 488 annexin V
线粒体健康
Membrane Asymmetry
--
488
V35116
Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue™ annexin V/SYTOX® AADvanced™
Membrane Asymmetry
坏死
--
405/488
A35136
Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO®-1 and 7-aminoactinomycin D
Membrane Asymmetry
坏死
--
405/488
V35123
Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant® DyeCycle™ Violet and SYTOX® AADvanced™
Chromatin Condensation
坏死
--
405/488
A35135

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