Fluorobrite-showcase

活细胞荧光成像是用于观察十分重要及生理学相关的生物学事件的一项基本技术。该技术面临的一个主要挑战在于,能否在不引起细胞损伤、光漂白或细胞健康异常变化的情况下实现微弱荧光基团的清晰成像。为了解决这个问题,我们成功开发出了FluoroBrite™ DMEM,它是一种基于DMEM配方的培养基,其背景荧光与PBS相当,与标准的不含酚红的DMEM培养基相比,其背景荧光降低了90%。FluoroBrite™ DMEM可提供常规细胞培养所需的营养,与常规细胞培养方案一样,只需在细胞培养时加入10%胎牛血清和4 mM L-谷氨酰胺或GlutaMAX即可。FluoroBrite™ DMEM 增强了荧光基团的信噪比,即使是最微弱的荧光也能被观察到,同时赋予细胞健康生长的环境。

  • 降低荧光背景,从而增强活细胞成像过程中的荧光信号
  • 以DMEM为基础,在活细胞成像过程中无需改变细胞培养环境,既可以保证细胞的健康生长,同时又可以完美实现活细胞荧光成像

来自于我们beta测试用户的评价

“简而言之,成像过程中所需的曝光时间更少,对比度更好,从而使得活细胞成像结果明显改善。”

Sylvain Costes (美国劳伦斯伯克力国家实验室)

性能数据

fluorobrite-data1

Figure 1. FluoroBrite™ DMEM has 10% of the background fluorescence emitted by standard phenol red–free DMEM and provides a 9-fold enhancement in signal-to-noise ratio. (A) Images of live epithelial cells that have been labeled with CellLight Golgi-GFP, BacMam 2.0 (Cat. No. C10592) and cultured in phenol red–free DMEM (Cat. No. 31053) or FluoroBrite™ DMEM with 10% fetal bovine serum. Cells in 10X images are co-labeled with CellLight Actin-RFP, BacMam 2.0 (Cat. No. C10583). Cells in 40X images (inset) are co-labeled with ER-Tracker™ Red (BODIPY TR Glibenclamide; Cat. No. E34250). (B) Fluorescence signal from PBS, FluoroBrite™ DMEM, and phenol red–free DMEM at 509 nm in response to excitation at 480 nm. (C) Fluorescent signal from dextran labeled with Alexa Fluor 488 dye (Cat. No. D22910) over background in PBS, FluoroBrite™ DMEM, and phenol red–free DMEM.

fluorobrite-data2

Figure 2. Cell lines cultured in FluoroBrite™ DMEM and standard DMEM display comparable long-term growth over multiple passages. Graphs display the average viable cell density (solid line; left y axis) and average percent viability (dotted line; right y axis) for three cell lines cultured for several passages in a standard phenol red–free DMEM (Cat. No. 31053) or FluoroBrite™ DMEM, both supplemented with 10% fetal bovine serum and and 4 mM GlutaMAX™. Note: the Sp2 suspension cell line was tested for long-term growth in FluoroBrite™ DMEM because of its known hypersensitivity to nutrient deficiencies.