The Neon Transfection System is a second-generation transfection system that uses an electronic pipette tip as a transfection chamber. The first model of this technology, the Microporator (MP-100), was commercialized by Digital Bio in 2006. The MP-100 was the first electroporation technology that avoided the use of a standard electroporation cuvette and thus achieved exceptional transfection efficiency and high cell viability in difficult to transfect cells.  Invitrogen acquired the Microporator MP-100 technology and following some enhancements to the user interface, launched the technology to the world-wide market under the name, "Neon Transfection System".

The Microporator Technology

The design and performance of the patented Microporator MP-100 electronic pipette transfection chamber was described by Kim et al..  The inventors described that the innovative design caused a remarkable increase in cell viability and transfection efficiency compared to traditional cuvette based electroporation systems.  The Microporation MP-100 has helped many researchers improve their transfection experiments. As a result, there are now more than 40 scientific publications that cite the successful use of the Microporator MP-100 for delivery of plasmid DNA and siRNA.

Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 23(9):1353–1360.

The Next Generation Electroporation Technology

Invitrogen developed the Neon Transfection System from the Microporator MP-100 platform, by updating the touch screen user interface, pre-programmed gradient optimization procedure, and generating additional cell type specific protocol database of cell-specific protocols. The Neon Transfection system kits (with buffers and Neon Pipette Tips) are fully compatible with the original Microporator MP-100.