由于背景值较高,克隆平末端和较长的 PCR 产物一直十分困难,而且重组体的产率通常很低。 我们独特的 Zero Background™ 技术使用 ccdB 致死基因(控制细胞死亡)来实现高效克隆。

ccdB 基因整合进入 Zero Background™ 载体的克隆位点。CcdB 蛋白毒素细菌促旋酶可导致宿主染色体降解和细胞死亡。 当一个插入片段连接至载体时,ccdB 基因被破坏,只有重组体克隆能够生长。 Zero Background™技术可消除较高的载体背景,得到接近 100% 的重组体,将您从筛选庞杂的背景克隆这一繁重的工作中解脱出来。

Zero Blunt Cloning Kits

Zero Blunt Cloning Kits now come with the addition of the ExpressLink™ T4 DNA Ligase into Zero Blunt Cloning Kits, ligation can now be performed at room temperature in only 5 minutes with reactions typically yielding >80% recombinants containing inserts.

Zero Blunt TOPO Cloning

Go from PCR to clone in just 5-minutes using Zero Blunt TOPO technology. Zero Blunt TOPO yields up to 95% clones with the correct insert and no ligase, post-PCR procedures, or PCR primers containing specific sequences are required.