5-Minute Cloning and positive selection with Zero Blunt TOPO

Zero Blunt TOPO technology provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has the Zero Blunt TOPO vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants so you can obtain up to 95% clones with correct insert.

The pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase.

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 The pCRBlunt II-TOPO vector features:

  • Contains the ccdB gene for positive selection
  • EcoRI sites flanking the PCR product insertion site for easy excision of inserts
  • 卡那霉素和 Zeocin™ 抗性基因可用于在 E. coli 中进行选择
  • SP6 promoter/primer site for in vitro RNA transcription and sequencing
  • M13 正向与反向引物位点,用于测序或 PCR 筛选
 

pCR-II Blunt TOPO vector

The pCRBlunt II-TOPO vector

Performance and value

Each Zero Blunt TOPO for subcloning Kit contains linearized and topoisomerase I-activated pCRBlunt II-TOPO Vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent or Electrocomp™ E. coli, S.O.C. Medium, and a supercoiled control plasmid. The Zero Blunt TOPO PCR Cloning Kit for Subcloning, without competent cells allows the flexibility of using your own strain for increased flexibility so competent cells and S.O.C. Medium are not provided.

In addition, a Zero Blunt TOPO Cloning Kit is available combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis.

克隆的 PCR 产物的应用 产品 Includes One Shot Competent E. coli 优点 Rxn number 目录号  
常见亚克隆 Zero Blunt TOPO PCR Cloning Kit (with pCR Blunt II TOPO vector)
  • Flexibility to use your own competent cell strain
20 450245 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Most popular kit
20 K280020 马上订购
MAX Efficiency DH5 α™T1 Phage Resistant (genotype)
  • Classic DH5 cloning strain
20 K282020 马上订购
TOP10 Electrocomp™ (genotype)
  • Electrocompetent cells for highest transformation efficiency
20 K286020 马上订购
Mach1™T1R (genotype)
  • 为生长快速的细胞,利于进行快速地质粒的小量提取
20 K283020 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Includes PureLink™ Quick Plasmid Miniprep Kit
20 K280002 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Most popular kit
40 K280040 马上订购
MAX Efficiency DH5 α™T1 Phage Resistant (genotype)
  • Classic DH5 cloning strain
40 K282040 马上订购
TOP10 Electrocomp™ (genotype)
  • Electrocompetent cells for highest transformation efficiency
40 K286040 马上订购