5-Minute Cloning and positive selection with Zero Blunt® TOPO®

Zero Blunt® TOPO® technology provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has the Zero Blunt® TOPO® vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants so you can obtain up to 95% clones with correct insert.

The pCR®Blunt II-TOPO® vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum® Pfx DNA Polymerase.

立即订购

 The pCR®Blunt II-TOPO® vector features:

  • Contains the ccdB gene for positive selection
  • EcoRI sites flanking the PCR product insertion site for easy excision of inserts
  • 卡那霉素和 Zeocin™ 抗性基因可用于在 E. coli 中进行选择
  • SP6 promoter/primer site for in vitro RNA transcription and sequencing
  • M13 正向与反向引物位点,用于测序或 PCR 筛选
 

pCR®-II Blunt TOPO® vector

The pCR®Blunt II-TOPO® vector

Performance and value

Each Zero Blunt® TOPO® for subcloning® Kit contains linearized and topoisomerase I-activated pCR®Blunt II-TOPO® Vector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot® Chemically Competent or Electrocomp™ E. coli, S.O.C. Medium, and a supercoiled control plasmid. The Zero Blunt® TOPO® PCR Cloning Kit for Subcloning, without competent cells allows the flexibility of using your own strain for increased flexibility so competent cells and S.O.C. Medium are not provided.

In addition, a Zero Blunt® TOPO® Cloning® Kit is available combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.

克隆的 PCR 产物的应用 产品 Includes One Shot® Competent E. coli 优点 Rxn number 目录号  
常见亚克隆 Zero Blunt® TOPO® PCR Cloning Kit (with pCR® Blunt II TOPO® vector)
  • Flexibility to use your own competent cell strain
20 450245 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Most popular kit
20 K280020 马上订购
MAX Efficiency® DH5 α™T1 Phage Resistant (genotype)
  • Classic DH5 cloning strain
20 K282020 马上订购
TOP10 Electrocomp™ (genotype)
  • Electrocompetent cells for highest transformation efficiency
20 K286020 马上订购
Mach1™T1R (genotype)
  • 为生长快速的细胞,利于进行快速地质粒的小量提取
20 K283020 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Includes PureLink™ Quick Plasmid Miniprep Kit
20 K280002 马上订购
TOP10 (genotype)
  • Classic TOP10 strain
  • Most popular kit
40 K280040 马上订购
MAX Efficiency® DH5 α™T1 Phage Resistant (genotype)
  • Classic DH5 cloning strain
40 K282040 马上订购
TOP10 Electrocomp™ (genotype)
  • Electrocompetent cells for highest transformation efficiency
40 K286040 马上订购