Life Technologies offers a collection of TaqMan™ Drug Metabolism Genotyping Assays for pharmacogenomics applications. This product provides 2,700 unique assays to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters. View the Drug Metabolism Genotyping Assay Index in Excel (908 KB) or Text (905 KB) format.

  • Select individual assays for your drug metabolism studies
  • Study single nucleotide polymorphisms (SNPs), multiple nucleotide polymorphisms (MNPs), and insertions/deletions (In/Dels) with the ease of use and flexibility in choosing your targets
  • Work confidently — our bioinformatics mapping, design, and in silico QC means you will study the right SNP
  • 95% coverage of PharmaADME core targets

TaqMan Drug Metabolism Genotyping Assays detect biologically important polymorphisms that reside in complex portions of the genome. These polymorphisms have been associated with certain diseases such as cancer, and have been shown to significantly impact drug efficacy. Where possible all assays have been mapped to the common public allele nomenclature. All TaqMan DME Genotyping Assays have proven performance across 180 unique DNA samples.

Product categories

Related products

Data Analysis Tools

Discrimination 5' nuclease assay to discriminate between 2 alleles of a specific SNP for use in genotyping studies.
Assay Components Each pre-formulated assay contains:
  • 2 unlabelled PCR primers: (forward and reverse (primers at 900 nM final concentration)
  • 1 VIC® dye – MGB labeled probe detects the Allele 1 sequence (probes at 200 nM final concentration)
  • 1 6FAM™ dye – MGB labeled probe detects the Allele 2 sequence (probes at 200 nM final concentration)
Reactions Sufficient for 750 5ul reactions in a 384-well plate 
Expiration Date 5 years from the date of manufacturing
Documentation Available to download at thermofisher.com/taqmanfiles :
  • Protocol for TaqMan® SNP Genotyping Assays products (pdf file)
  • Reference Guide: TaqMan® Drug Metabolism Genotyping Assays                            
  • Product Insert (pdf file)
  • Assay Information File (tab delimited text format)
  • Understanding Your Assay Information (text file)
  • Safety Data Sheet (PDF)
Assay Information File Includes sales order number, part number, assay ID, probe and primer concentrations, and Context Sequence. Also includes NCBI dbSNP ID, chromosome location, cytogenic location, gene association, SNP type, and Applied Biosystems® minor allele frequencies, if applicable.

The Context Sequence is the nucleotide sequence surrounding the SNP site and is provided in the (+) genome strand orientation relative to the NCBI reference genome. The SNP alleles are included in brackets, where the order of the alleles corresponds to the association with probe reporter dyes, where [Allele 1 = VIC® dye / Allele 2 = FAM™ dye].
Tracking/Identification
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

Manuals & Protocols

Protocol: TaqMan® Drug Metabolism Genotyping Assays
Reference Guide: TaqMan® Drug Metabolism Genotyping Assays
Quick Reference Card: TaqMan® Genotyping Master Mix

Product Literature

Drug Metabolism Genotyping Assay Index: (XLS 908 KB) | (TXT 905 KB)
Poster: Coverage of the PharmaADME Consortium Core Marker List by TaqMan® DME and Copy Number Assays
Application Notes: TaqMan® Drug Metabolism Genotyping Assays for Triallelic SNPs
Bulletin: TaqMan® Drug Metabolism Genotyping Assays
Data Sheet: TaqMan® Drug Metabolism Genotyping Assays
Poster: Annotation of Polymorphisms in Drug Metabolizing Enzymes
How Well do the HapMap SNPs “Tag” Functional Variants in Drug Metabolizing Enzyme Genes?

Product categories

Related products

Data Analysis Tools

Discrimination 5' nuclease assay to discriminate between 2 alleles of a specific SNP for use in genotyping studies.
Assay Components Each pre-formulated assay contains:
  • 2 unlabelled PCR primers: (forward and reverse (primers at 900 nM final concentration)
  • 1 VIC® dye – MGB labeled probe detects the Allele 1 sequence (probes at 200 nM final concentration)
  • 1 6FAM™ dye – MGB labeled probe detects the Allele 2 sequence (probes at 200 nM final concentration)
Reactions Sufficient for 750 5ul reactions in a 384-well plate 
Expiration Date 5 years from the date of manufacturing
Documentation Available to download at thermofisher.com/taqmanfiles :
  • Protocol for TaqMan® SNP Genotyping Assays products (pdf file)
  • Reference Guide: TaqMan® Drug Metabolism Genotyping Assays                            
  • Product Insert (pdf file)
  • Assay Information File (tab delimited text format)
  • Understanding Your Assay Information (text file)
  • Safety Data Sheet (PDF)
Assay Information File Includes sales order number, part number, assay ID, probe and primer concentrations, and Context Sequence. Also includes NCBI dbSNP ID, chromosome location, cytogenic location, gene association, SNP type, and Applied Biosystems® minor allele frequencies, if applicable.

The Context Sequence is the nucleotide sequence surrounding the SNP site and is provided in the (+) genome strand orientation relative to the NCBI reference genome. The SNP alleles are included in brackets, where the order of the alleles corresponds to the association with probe reporter dyes, where [Allele 1 = VIC® dye / Allele 2 = FAM™ dye].
Tracking/Identification
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

Manuals & Protocols

Protocol: TaqMan® Drug Metabolism Genotyping Assays
Reference Guide: TaqMan® Drug Metabolism Genotyping Assays
Quick Reference Card: TaqMan® Genotyping Master Mix

Product Literature

Drug Metabolism Genotyping Assay Index: (XLS 908 KB) | (TXT 905 KB)
Poster: Coverage of the PharmaADME Consortium Core Marker List by TaqMan® DME and Copy Number Assays
Application Notes: TaqMan® Drug Metabolism Genotyping Assays for Triallelic SNPs
Bulletin: TaqMan® Drug Metabolism Genotyping Assays
Data Sheet: TaqMan® Drug Metabolism Genotyping Assays
Poster: Annotation of Polymorphisms in Drug Metabolizing Enzymes
How Well do the HapMap SNPs “Tag” Functional Variants in Drug Metabolizing Enzyme Genes?

Resources
Support

Description

Life Technologies offers a collection of TaqMan® Drug Metabolism Genotyping Assays for pharmacogenomics applications. This product provides 2,700 unique assays to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters. View the Drug Metabolism Genotyping Assay Index in Excel (908 KB) or Text (905 KB) format.

  • Select individual assays for your drug metabolism studies
  • Study single nucleotide polymorphisms (SNPs), multiple nucleotide polymorphisms (MNPs), and insertions/deletions (In/Dels) with the ease of use and flexibility in choosing your targets
  • Work confidently — our bioinformatics mapping, design, and in silico QC means you will study the right SNP
  • 95% coverage of PharmaADME core targets
TaqMan® Drug Metabolism Genotyping Assays detect biologically important polymorphisms that reside in complex portions of the genome. These polymorphisms have been associated with certain diseases such as cancer, and have been shown to significantly impact drug efficacy. Where possible all assays have been mapped to the common public allele nomenclature. All TaqMan® DME Genotyping Assays have proven performance across 180 unique DNA samples.

Specifications

General Specifications

Discrimination:5' nuclease assay to discriminate between 2 alleles of a specific SNP for use in genotyping studies.
Assay Components:Each pre-formulated assay contains:
  • 2 unlabelled PCR primers: (forward and reverse (primers at 900 nM final concentration)
  • 1 VIC® dye – MGB labeled probe detects the Allele 1 sequence (probes at 200 nM final concentration)
  • 1 6FAM™ dye – MGB labeled probe detects the Allele 2 sequence (probes at 200 nM final concentration)
Reactions:Sufficient for 750 5ul reactions in a 384-well plate
Expiration Date:5 years from the date of manufacturing
Documentation:Each assay shipment contains a CD-ROM disc containing:
  • Protocol for TaqMan® SNP Genotyping Assays products (pdf file)
  • Reference Guide: TaqMan® Drug Metabolism Genotyping Assays
  • Product Insert (pdf file)
  • Assay Information File (tab delimited text format)
  • Understanding Your Assay Information (text file)
  • Safety Data Sheet (PDF)
Assay Information File:Includes sales order number, part number, assay ID, , probe and primer concentrations, allele nomenclature and Context Sequence. Also includes NCBI dbSNP ID, chromosome location, cytogenic location, gene association, SNP type, and Applied Biosystems minor allele frequencies.

The Context Sequence is the nucleotide sequence surrounding the SNP site and is provided in the (+) genome strand orientation relative to the NCBI reference genome. The SNP alleles are included in brackets, where the order of the alleles corresponds to the association with probe reporter dyes, where [Allele 1 = VIC® dye / Allele 2 = FAM™ dye].
Tracking/Identification:
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes

Shipping and Storage

Shipped at ambient temperature.
Store at -15°C to 25°C in the dark, and minimize freeze-thaw cycles.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

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