TaqMan MicroRNA Assays are innovative tools for miRNA research—from isolation through discovery, profiling, quantitation, validation, and functional analysis.

TaqMan MicroRNA Assays employ a novel target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan Assay–based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.

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Use our Search Tool below to find your assays.

miRBase v.20 update: Our assay catalog is currently being updated to miRBase v.20. If you cannot find an assay for a newly introduced miRNA, it will be made available shortly.

下载: Complete list of TaqMan MicroRNA Assays

下载: Protocol for multiplexing RT and preamplification reactions with TaqMan MicroRNA Assays

请注意:
For miRNAs not included in miRBase and for any other Small RNAs, we offer custom designed Custom TaqMan Small RNA Assay.
For noncoding human, mouse, or rat transcripts longer than 200 nucleotides, we offer TaqMan Non-coding RNA Assays.

Use our Search Tool below to find the single tube assays you need.

Assay Search Tool:

TaqMan Pri-miRNA分析

TaqMan Pri-miRNA Assays for detecting and measuring primary miRNAs from human, mouse, or rat species.


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TaqMan非编码RNA分析

TaqMan Non-coding RNA Assays for reliable detection and quantitation of noncoding human, mouse, or rat transcripts longer than 200 nucleotides.

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定制TaqMan小RNA分析

 

Custom assays to quantitate small RNA are ideal to quantitate your small RNA when a predesigned assay is unavailable—all you need is the sequence of interest.

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Plated Formats

Plated Formats

96-Well Plates

Order your TaqMan MicroRNA Assays preplated into Fast or standard 96-well plates with the TaqMan Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

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384-Well Microfluidic Cards

384-well cards are ideal for profiling studies across a small number of samples (up to 8 samples per card) or with limited sample material (each reaction requires only 2 µL of sample) and are compatible with Applied Biosystems ViiA™ 7 and 7900HT Real-Time PCR Systems.

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384-Well Plates

Order your TaqMan MicroRNA Assays preplated into Fast or standard 384-well plates with the TaqMan Custom Plating Service. Perfect for analyzing a large number of samples for a few target miRNAs or noncoding RNAs, or the converse, evaluating a few samples for a large number of targets.

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OpenArray Plates

These plates accommodate 3,072 reactions at once, delivering the high-throughput capabilities required for large profiling studies. Designed for use with the Applied Biosystems OpenArray Real-Time PCR System and the new QuantStudio™ 12K Flex Real-Time PCR System.

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QuantStudio 12K Flex Real-Time PCR System

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