TaqMan Advanced miRNA Assays

TaqMan advanced miRNA分析采用qPCR技术,可对成熟miRNA进行高敏感性和高特异性定量。通过配合使用TaqMan™ advanced miRNA cDNA合成试剂盒,该解决方案可提供简单的前期工作流程,适用于分析单个样本中的多个miRNA靶标或低RNA水平的血清和血浆样本,并具有出色的灵敏度和特异性。

  • 通用的逆转录 (RT) ——所有TaqMan advanced miRNA分析均使用同一种逆转录步骤
  • 灵敏度——在用于cDNA合成的起始样本中,低至60拷贝数的miRNA都可检测到
  • 特异性——只检测成熟miRNA和区分高度相似的靶标
  • 样本输入量小——可对低至1 pg总RNA或2 µL血浆中的成熟miRNA进行检测和定量
  • 兼容性好——与人体血清、血浆和组织等生物液体样本兼容


Already know your target of interest?

Search for a pre-designed TaqMan Advanced miRNA Assay using the tool below:

Looking to design your own assay? Email specialty_oligos@thermofisher.com to request a design for a custom TaqMan Advanced miRNA Assay when a pre-designed assay is not offered.

Availbility & sizes: Inventoried assays are pre-packaged in "Small" scale and are ready to ship.

taqman-array-cards-plate

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format

Enable highly sensitive and specific quantification of mature miRNAs using qPCR in higher throughputs.

Search for pre-designed arrays ›
Design your own Custom TaqMan Array Advanced miRNA Card ›


Taqman Advanced miRNA Product Selection Guide

Format

Recommended TaqMan assay/array

Individual qPCR Assays

New TaqMan Advanced miRNA Assays
Comprehensive collection of pre-designed TaqMan qPCR assays for human, mouse and rat species

All pre-designed assays are inventoried and ship out in 1-2 days

Search for pre-designed assays ›

96-well plate

TaqMan Advanced miRNA  96 well array plates
Comprehensive collection of pre-plated, pre-designed panels available in both fast and standard 96 well formats.

Search for pre-designed 96 well plates ›

384-well microfluidic TaqMan Array card

TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 380 miRNA targets from as little as 1 ng of total RNA

Search for predesigned 384-well arrays ›
Design your own custom 384-well array ›

OpenArray plate TaqMan Advanced miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays)

Contact: QuantStudioFrontDesk@lifetech.com
Custom 384 well plates Contact: specialty_plates@thermofisher.com
工作流程
mirVana™ miRNA isolation kits

mirVana miRNA分离试剂盒

mirVana™ miRNA分离试剂盒采用快速的方法,可以从组织和细胞中分离包括小RNA在内的总RNA,如miRNA、小干扰 RNA (siRNA) 和核内小分子RNA (snRNA)。

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan高级miRNA cDNA合成试剂盒

TaqMan™ 高级miRNA cDNA合成试剂盒适用于从一个样本的全部成熟miRNA中制备cDNA模板。

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp通用扩增

miR-Amp采用通用的引物和预混液,均匀地增加每个靶标的cDNA量。 TaqMan™高级miRNA cDNA合成试剂盒包含miR-Amp试剂。

Applied Biosystems™ real-time PCR instruments

Applied Biosystems实时定量PCR仪

您可以使用我们的Applied Biosystems实时荧光定量PCR平台,实现真正的价值,获得极佳的性能、可靠性和世界级支持。产品设计兼容TaqMan Assay,使用灵活,不受限制于实时荧光定量化学反应的选择,使您可以轻松上手。

mirVana™ miRNA isolation kits

mirVana miRNA分离试剂盒

mirVana™ miRNA分离试剂盒采用快速的方法,可以从组织和细胞中分离包括小RNA在内的总RNA,如miRNA、小干扰 RNA (siRNA) 和核内小分子RNA (snRNA)。

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan高级miRNA cDNA合成试剂盒

TaqMan™ 高级miRNA cDNA合成试剂盒适用于从一个样本的全部成熟miRNA中制备cDNA模板。

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp通用扩增

miR-Amp采用通用的引物和预混液,均匀地增加每个靶标的cDNA量。 TaqMan™高级miRNA cDNA合成试剂盒包含miR-Amp试剂。

Applied Biosystems™ real-time PCR instruments

Applied Biosystems实时定量PCR仪

您可以使用我们的Applied Biosystems实时荧光定量PCR平台,实现真正的价值,获得极佳的性能、可靠性和世界级支持。产品设计兼容TaqMan Assay,使用灵活,不受限制于实时荧光定量化学反应的选择,使您可以轻松上手。


Additional information

种类 TaqMan advanced miRNA分析适用于人miRNA。我们将持续增加miRNA分析的数量,使其与Sanger miRBase序列数据库一致。
组成

TaqMan advanced miRNA分析为单管包装,含有特异性预先配好的TaqMan Assay(TaqMan探针和PCR引物对)。

供货规格
产品 Catalog no. 反应次数
TaqMan advanced miRNA分析 A25576 250次qPCR反应 (20 μL)
运输和储存

常温运输。
存放在 –15ºC to –25ºC.

白皮书:常温运输可减少环境影响,保持质量和稳定性

种类 TaqMan advanced miRNA分析适用于人miRNA。我们将持续增加miRNA分析的数量,使其与Sanger miRBase序列数据库一致。
组成

TaqMan advanced miRNA分析为单管包装,含有特异性预先配好的TaqMan Assay(TaqMan探针和PCR引物对)。

供货规格
产品 Catalog no. 反应次数
TaqMan advanced miRNA分析 A25576 250次qPCR反应 (20 μL)
运输和储存

常温运输。
存放在 –15ºC to –25ºC.

白皮书:常温运输可减少环境影响,保持质量和稳定性