Custom TaqMan Array Cards
The Custom TaqMan® Array Card is a 384-well microfluidic card designed to perform 384 simultaneous real-time PCR (qPCR) reactions without using liquid-handling robots or multichannel pipettors to fill the card. This format allows for 1–8 samples to be run in parallel against 12–384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the card.
- Design a Custom TaqMan® Array Card to meet your specific need
- Load 384 wells in less than five minutes without liquid-handling robots or multi-channel pipettors
- Validate all of the “interesting” hits from a microarray experiment quickly and economically
- Standardize the screening of gene panels across many samples and laboratories
How to Order
Select the TaqMan Array Card format for your experiment, and have it pre-loaded with your selected assays in each reaction well. Choose 12–384 target assays from over 50,000 inventoried TaqMan Gene Expression Assays covering human, mouse, and rat genes. Ordering is easy with the Custom TaqMan Array Card configuration tool to help you search and select genes and assays for real-time PCR quantification.
TaqMan® Array Cards are ideal for screening biomarkers and toxicology panels and for analyzing pathways, target classes, and complete disease sets. TaqMan® Array Cards provide standardized results with low variability across many users and laboratories.
Using the TaqMan® Array Card is simple. Each card has 8 sample-loading ports which each connect to a set of 48 reaction wells. Simply pipette your sample pre-mixed with TaqMan® Universal Master Mix II into each port and centrifuge. Within minutes, your card is ready to run on an Applied Biosystems® ViiA™ 7 or 7900HT Fast Real-Time PCR System.
TaqMan® Array Cards are great tools for validating the 10's or 100's of “interesting” hits that come from microarray experiments because TaqMan® Array Cards can be customized to include up to 384 of those hits in one easy-to-use card. Using individual assays—even SYBR® Green Dye I-based assays—make validations of this magnitude unmanageable and expensive.
- Download your TaqMan™ documentation files
- Video: Preparing a TaqMan™ Array Card
- Video: How to analyze TaqMan™ Array Card data
User Bulletin: Running TaqMan™ Custom Arrays on 7900HT Real-Time PCR Systems
Protocol: Custom TaqMan™ PreAmp Pools
Product Bulletin: TaqMan™ Array Cards
Centrifuge Compatibility Table
Poster: Customizable qPCR assay for respiratory tract pathogen detection