QuantStudio 5 Real-Time PCR System

Take charge of how you create and manage your real-time PCR results

The QuantStudio 5 Real-Time PCR System is ideal for all levels of users who need a high-performance instrument with features for maximum experiment control. With built-in security features, locked workflows, user account management and options for Web browser-based or desktop analysis, the QuantStudio 5 Real-Time PCR System helps connect you to your data more easily than ever before and offers uncompromising performance and quality.

Request a quote      Try the demo


帮助您创建和管理实时荧光定量PCR结果

用于不同水平的用户,具有最佳的实验参照系统。QuantStudio 5实时荧光定量PCR系统内置了安全功能、固定的流程、用户账户管理及基于Web浏览器或桌面分析的软件选项,能够助您以前所未有的便利访问数据,并提供绝佳的性能和质量。

Request a quote  Try the demo

QuantStudio 5实时荧光定量PCR系统提供有0.1或0.2 mL反应体积的96孔热循环模块或384孔的热循环模块。

Contact us
Telephone: 800 327 3002, option 4, then option 3 (toll-free in the US only)
Telephone 00 800 5345 5345, option 3 (toll-free in Europe, Middle East and South Africa only)

Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.

Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.

Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.

Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.

Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.

Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:

  • Remotely monitor your runs and access data anytime, anywhere*
  • Analyze sophisticated data sets quickly
  • Store results in a secure data storage space
  • Share and collaborate with colleagues at your institution or around the world

For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.

Learn more

*Where the Web is accessible

Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.

Precise quantification with 1.5-fold discrimination

Figure 5-A

A

Figure 5-B

B

Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.

Excellent reproducibility and 10-log dynamic range

Figure 6

Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.


The QuantStudio 5 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 5 system offers 96- well and 384-well format options, allowing for a broader range of detection chemistries and assay multiplexing. The 96-well format has six excitation filters (450–680 nm) and six emission filters (500–730 nm), and the 384-well format has five excitation filters (450–650 nm) and five emission filters (500–700 nm), calibrated form FAM/SYBR Green, VIC, ABY/NED/TAMRA, Jun/ROX dyes, and Cy5/Mustang Purple dyes.

Melt curve analysis

Figure 7

Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).

Genotyping analysis

Figure 9

Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.

Multiplex gene expression

WPR2102_Fig8

Figure 5. Multiplex reaction with four targets plus passive reference. Whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR) amplified under Fast run conditions using Applied Biosystems TaqMan. Multiplex Master Mix with Mustang Purple passive reference dye. Targets and labels: FZD1 labeled with FAM dye, APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled with JUN dye.

  On-site service plans
Service plans at a glance AB Maintenance AB Assurance AB Complete
On-site response time Target 2 business days* 2 business days* Next business day*
Scheduled on-site planned maintenance (PM) Yes Yes Yes
Remote diagnostics Yes Yes Yes
Parts, labor, and travel for repair included   Yes Yes
Computer repair and replacement included   Yes Yes
Priority access to Tech Support
Mon–Fri, 9 a.m.—5 p.m. local time
  Yes Yes
Requalification post-PM and critical repairs     Yes
Field Application Scientist consultation     Yes

*Availability limited in some geographic areas.

QuantStudio Family of Real-Time PCR and Digital PCR Systems
Product brochure: QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
Your Innovative Research: QuantStudio 5 Real-Time PCR System
Application Note: QuantStudio 3 and 5 Real-Time PCR Systems
Your Innovative Research: Expression of oncogenic microRNAs
Application Note: Protein Thermal Shift Technology

Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.

Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.

Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.

Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.

Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.

Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:

  • Remotely monitor your runs and access data anytime, anywhere*
  • Analyze sophisticated data sets quickly
  • Store results in a secure data storage space
  • Share and collaborate with colleagues at your institution or around the world

For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.

Learn more

*Where the Web is accessible

Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.

Precise quantification with 1.5-fold discrimination

Figure 5-A

A

Figure 5-B

B

Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.

Excellent reproducibility and 10-log dynamic range

Figure 6

Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.


The QuantStudio 5 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 5 system offers 96- well and 384-well format options, allowing for a broader range of detection chemistries and assay multiplexing. The 96-well format has six excitation filters (450–680 nm) and six emission filters (500–730 nm), and the 384-well format has five excitation filters (450–650 nm) and five emission filters (500–700 nm), calibrated form FAM/SYBR Green, VIC, ABY/NED/TAMRA, Jun/ROX dyes, and Cy5/Mustang Purple dyes.

Melt curve analysis

Figure 7

Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).

Genotyping analysis

Figure 9

Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.

Multiplex gene expression

WPR2102_Fig8

Figure 5. Multiplex reaction with four targets plus passive reference. Whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR) amplified under Fast run conditions using Applied Biosystems TaqMan. Multiplex Master Mix with Mustang Purple passive reference dye. Targets and labels: FZD1 labeled with FAM dye, APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled with JUN dye.

  On-site service plans
Service plans at a glance AB Maintenance AB Assurance AB Complete
On-site response time Target 2 business days* 2 business days* Next business day*
Scheduled on-site planned maintenance (PM) Yes Yes Yes
Remote diagnostics Yes Yes Yes
Parts, labor, and travel for repair included   Yes Yes
Computer repair and replacement included   Yes Yes
Priority access to Tech Support
Mon–Fri, 9 a.m.—5 p.m. local time
  Yes Yes
Requalification post-PM and critical repairs     Yes
Field Application Scientist consultation     Yes

*Availability limited in some geographic areas.

QuantStudio Family of Real-Time PCR and Digital PCR Systems
Product brochure: QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
Your Innovative Research: QuantStudio 5 Real-Time PCR System
Application Note: QuantStudio 3 and 5 Real-Time PCR Systems
Your Innovative Research: Expression of oncogenic microRNAs
Application Note: Protein Thermal Shift Technology

可快速上手—系统在出厂时已校准,具有易用软件包,可快速安装并立即使用。

提供方便的标准运行步骤与合规性—用户账户管理、固定程序模板、反应质控(QC)反馈、耗材的QC可追踪能力,以及软件暂停特性等等都能够为用户的实验数据提供良好参照。可进行实时的数据标记语言(Real-Time Data Mark-Up Language,RDML)输出,兼容MIQE准则。

实验效率高,节省时间 —VeriFlex™技术具有3个独立的精确数码温控区域供PCR优化,快速热循环可使您在30分钟内得到实验结果。

您值得信赖的最高多重分析能力 —OptiFlex®技术具有6色荧光通道,支持21种荧光组合,高亮度白光半导体光源能够帮助用户优化孔与孔之间,仪器与仪器之间的精确度,在QuantStudio® 5 96孔模块系统上实现多重反应的通量最大化。

通过一款具有触摸屏的实时定量PCR系统,为用户提供接近移动设备的现代化操作经验,您进而能够以前所未有的便捷性地访问数据;同时本设备中也可选择基于网络浏览器的软件选项,用户可通过Thermo Fisher Cloud进行访问。通过云服务,您能够:

  • 远程监控您的反应,并在任何时间和地点对数据进行访问*
  • 快速分析复杂的数据集合
  • 在安全的数据存储空间保存结果
  • 与机构内或世界各地的同事分享和沟通

Thermo Fisher Cloud具有高行业标准的安全性,可保障您工作内容的安全,让您高枕无忧。我们已经与Amazon Web Services (AWS) 合作创建强大的平台。

了解更多

*可接入网络之处

  Repair Center service plans On-site service plans
Service plans at a glance Applied Biosystems Repair Center Support Plus Applied Biosystems Repair Center Support Plus Care Applied Biosystems Maintenance Plus Applied Biosystems Assurance Applied Biosystems Complete
On-site response time     Target 2 business days* Guaranteed 2 business days* Guaranteed next business day*
Scheduled on-site planned maintenance (PM)   Yes Yes Yes Yes
Remote diagnostics Yes Yes Yes Yes Yes
Parts, labor, and travel for repair included Yes Yes Yes Yes Yes
Computer repair and replacement included Yes Yes 10% discount optional add-on in selected regions Yes Yes
Priority access to Tech Support
Mon–Fri, 8 a.m.—5 p.m. local time
Yes Yes   Yes Yes
Priority access to Remote Service Engineer Yes Yes   Yes Yes
Requalification post-PM and critical repairs         Yes
Field Application Scientist consultation         Yes
Loaner instrument issued during repair (Repair Center plans only) Yes Yes      

*Instrument Management not available in all regions.

产品手册:QuantStudio 3和QuantStudio 5实时荧光定量PCR系统

可快速上手—系统在出厂时已校准,具有易用软件包,可快速安装并立即使用。

提供方便的标准运行步骤与合规性—用户账户管理、固定程序模板、反应质控(QC)反馈、耗材的QC可追踪能力,以及软件暂停特性等等都能够为用户的实验数据提供良好参照。可进行实时的数据标记语言(Real-Time Data Mark-Up Language,RDML)输出,兼容MIQE准则。

实验效率高,节省时间 —VeriFlex™技术具有3个独立的精确数码温控区域供PCR优化,快速热循环可使您在30分钟内得到实验结果。

您值得信赖的最高多重分析能力 —OptiFlex®技术具有6色荧光通道,支持21种荧光组合,高亮度白光半导体光源能够帮助用户优化孔与孔之间,仪器与仪器之间的精确度,在QuantStudio® 5 96孔模块系统上实现多重反应的通量最大化。

通过一款具有触摸屏的实时定量PCR系统,为用户提供接近移动设备的现代化操作经验,您进而能够以前所未有的便捷性地访问数据;同时本设备中也可选择基于网络浏览器的软件选项,用户可通过Thermo Fisher Cloud进行访问。通过云服务,您能够:

  • 远程监控您的反应,并在任何时间和地点对数据进行访问*
  • 快速分析复杂的数据集合
  • 在安全的数据存储空间保存结果
  • 与机构内或世界各地的同事分享和沟通

Thermo Fisher Cloud具有高行业标准的安全性,可保障您工作内容的安全,让您高枕无忧。我们已经与Amazon Web Services (AWS) 合作创建强大的平台。

了解更多

*可接入网络之处

  Repair Center service plans On-site service plans
Service plans at a glance Applied Biosystems Repair Center Support Plus Applied Biosystems Repair Center Support Plus Care Applied Biosystems Maintenance Plus Applied Biosystems Assurance Applied Biosystems Complete
On-site response time     Target 2 business days* Guaranteed 2 business days* Guaranteed next business day*
Scheduled on-site planned maintenance (PM)   Yes Yes Yes Yes
Remote diagnostics Yes Yes Yes Yes Yes
Parts, labor, and travel for repair included Yes Yes Yes Yes Yes
Computer repair and replacement included Yes Yes 10% discount optional add-on in selected regions Yes Yes
Priority access to Tech Support
Mon–Fri, 8 a.m.—5 p.m. local time
Yes Yes   Yes Yes
Priority access to Remote Service Engineer Yes Yes   Yes Yes
Requalification post-PM and critical repairs         Yes
Field Application Scientist consultation         Yes
Loaner instrument issued during repair (Repair Center plans only) Yes Yes      

*Instrument Management not available in all regions.

产品手册:QuantStudio 3和QuantStudio 5实时荧光定量PCR系统

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