TaqMan Real-Time PCR Master Mixes
TaqMan™ Master Mixes contain buffer, dNTPs, passive reference dye, thermostable hot-start DNA polymerase, and other components formulated for reliable TaqMan™ Assay-based real-time PCR. Just add your sample and TaqMan Assay, and start your reactions.
Which TaqMan master mix is right for you?
|Order now||Order now|
Gene Expression (Fast or Standard mode)
Genotyping and copy number variation (CNV) (Fast and Standard mode)
|TaqMan™ Fast Advanced Master Mix||TaqPath™ ProAmp™ Master Mix (GPR*)|
|Price / 20 µL reaction||$0.81–$0.95||$0.58–$0.85|
|Application examples||Gene expression for mRNA & small RNA||SNP genotyping, mutation detection, crude lysate samples, and CNV|
|Cycling/run time||0:40||0:50 (fast) / 1:20 (standard)|
|UDG to prevent DNA carryover contamination||✓||✓|
|ROX or Mustang purple reference dye to normalize signal & ensure data integrity||ROX||ROX or Mustang purple (mulitplex up to 4 targets)|
|Multiplex||Up to 2 targets (see Multiplex Master Mix for 4 target gene expression multiplex)||Up to 4 targets|
|Universal cycling for assay to assay consistency||✓||✓|
|Benchtop stability||72 hr||72 hr|
|Tolerant to PCR inhibitors||✓||✓|
While Applied Biosystems™ TaqMan™ Universal Master Mix II can be used for all applications, TaqPath™ ProAmp™ Master Mix has been specifically designed and will provide the best performance for genotyping applications (e.g., CNV, DME, and SNP).
* For laboratory use. General purpose reagent.
TaqMan™ PreAmp Master Mix is designed to facilitate analysis of limited-quantity samples, including single cells. Use the mix to expand small amounts of cDNA by pre-amplification without introducing amplification bias to the sample. Pre-amplification enables you to stretch your precious sample for use in many more real-time PCR reactions than you could otherwise run.
TaqMan™ Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this master mix to analyze environmental, food, and other challenging samples.
TaqMan™ Genotyping Master Mix is specifically formulated for detection of copy number, DME’s, and SNPs. Use this mix for the best performance for genotyping applications.
TaqMan™ Fast Environmental Master Mix Beads have been designed to match or exceed the performance of standard (not fast) real-time PCR master mixes, delivering shorter run times than these master mixes (<40 minutes in fast mode) with an easy-to-use, lyophilized bead format. These beads are available with or without an Applied Biosystems™ VIC™ dye–labeled internal positive control (IPC).
For testing of Food and Environmental samples only.