First-Strand cDNA Synthesis Kit for Real-Time PCR
Choosing the correct first-strand gDNA synthesis kit is critical to obtaining high yields of cDNA from a wide range of input RNAs and improved RT-qPCR assays. From a value choice first-strand cDNA synthesis kit to the best performance in master mix with integrated gDNA removal, you have a choice to select the Invitrogen™ or Applied Biosystems™ cDNA synthesis kit to meet your research needs.
Which cDNA synthesis kits for RT-qPCR is right for you?
|Order now||Order now||Order now|
|Goal||Highest performance & convenience for RT-qPCR||Highest performance with effective removal of gDNA||Value choice for commonly used RNA templates|
|Product||SuperScript IV VILO Master Mix
||SuperScript IV VILO Master Mix with ezDNase
||High-Capacity cDNA Reverse Transcription Kit
|Reverse transcriptase||Invitrogen™ SuperScript™ IV||SuperScript IV||Invitrogen™ MultiScribe™ MuLV|
|Optimal reaction temp||50°C||50°C||37°C|
||10 min||12 min (10 min for RT + 2 min gDNA removal)||120 min|
|Reduced RNase H activity||Yes||Yes||No|
|Primers included||Random primers & Oligo(dT)20||Random primers & Oligo(dT)20||Random primers|
|Integrated gDNA removal||No||Yes||No|
|Protocol||Download protocol||Download protocol||Download protocol|
Other First-Strand cDNA Synthesis Kits for RT-qPCR