An encyclopedia of articles about protein analysis laboratory techniques

Pierce Protein Methods Library

Originally created to support Thermo Scientific Pierce products, this continually expanded and updated library is a valued resource for new and experienced researchers alike. Are you new to the protein biology laboratory and need a basic introduction to protein analysis techniques? Are you a seasoned scientist considering how to optimize an experiment involving protein expression, purification or detection? Pierce Protein Methods can help you. In addition, this educational content will assist you in choosing appropriate products from our portfolio to successfully accomplish your experiments.


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Protein Method Article Title Protein Method Categories Subtopics
An Overview of Cell Lysis and Protein Extraction Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Traditional Methods of Cell Lysis Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Detergents for Cell Lysis and Protein Extraction Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Lysis Solutions Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Cell Fractionation and Organelle Isolation Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Protease and Phosphatase Inhibitors Cell Lysis and Fractionation Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
An Overview of Affinity Purification Protein Purification Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Covalent Immobilization of Affinity Ligands Protein Purification Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
GST-tagged Proteins–Production and Purification Protein Purification Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
His-tagged Proteins–Production and Purification Protein Purification Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Overview of the Immunoprecipitation (IP) Technique Protein Purification Protein Isolation and Purification, Protein Assays and Analysis,
An Overview of Buffer Exchange Dialysis, Desalting, and Concentration Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Dialysis Methods for Protein Research Dialysis, Desalting, and Concentration Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
Desalting and Gel Filtration Chromatography Dialysis, Desalting, and Concentration Protein Isolation and Purification, Protein Assays and Analysis, Protein Mass Spectrometry
An Overview of Protein Assays Protein Assays Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Chemistry of Protein Assays Protein Assays Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein Assay Data Analysis Protein Assays Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
Protein-Protein Variation of Protein Assays Protein Assays Protein Assays and Analysis, Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,
An Overview of Electrophoresis Protein Gel Electrophoresis Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Protein Gel Stains Protein Gel Electrophoresis Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting
An Overview of Detection Probes Detection Probes, ELISA, Western Blotting Western Blotting, Protein Assays and Analysis,
Introduction to Secondary Antibodies Detection Probes, ELISA, Western Blotting Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Enzyme Probes Detection Probes, ELISA, Western Blotting Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Avidin-Biotin Interaction Detection Probes, ELISA, Western Blotting, Protein Purification Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Fluorescent Probes Detection Probes, Immunohistochemistry Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Luciferase Reporters Reporter Gene Assays Protein Assays and Analysis
An Overview of Western Blotting Western Blotting Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Chemiluminescent Western Blotting Western Blotting Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Stripping and Reprobing Western Blots Western Blotting , Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis,
Fluorescent Western Blotting Western Blotting Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis, Antibodies and Proteins
Blocking Buffers for Western Blot and ELISA Western Blotting Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting, Protein Assays and Analysis
Western Blot Transfer Methods Western Blotting Protein Gel Electrophoresis, Western Blotting,
Chromogenic Western Blotting Substrates Western Blotting Protein Gel Electrophoresis, Western Blotting
Western Blot Video Tutorials Western Blotting Protein Gel Electrophoresis, Western Blotting
An Overview of ELISA ELISA Protein Assays and Analysis, Antibodies and Proteins
ELISA Development and Optimization ELISA Protein Assays and Analysis, Antibodies and Proteins
Spike-and-Recovery and Linearity-of-Dilution Assessment for ELISA ELISA Protein Assays and Analysis, Antibodies and Proteins
Factors Affecting Signal Generation in ELISA ELISA Protein Assays and Analysis, Antibodies and Proteins
ELISA Troubleshooting Guide ELISA Protein Assays and Analysis, Antibodies and Proteins
ELISA Data Analysis ELISA Protein Assays and Analysis, Antibodies and Proteins
An Overview of Immunohistochemistry Immunohistochemistry Antibodies and Proteins
Immunohistochemistry vs Immunocytochemistry Immunohistochemistry Antibodies and Proteins
Fixation Strategies and Formulations Immunohistochemistry Antibodies and Proteins
Paraffin Removal and Antigen Retrieval Immunohistochemistry Antibodies and Proteins
Methods to Block Endogenous Detection Immunohistochemistry Antibodies and Proteins
Blocking Strategies for IHC Immunohistochemistry Antibodies and Proteins
IHC Immunodetection Immunohistochemistry Antibodies and Proteins
Avidin-Biotin Complex Method for IHC Detection Immunohistochemistry Antibodies and Proteins
Immunofluorescence Method for IHC Detection Immunohistochemistry Antibodies and Proteins
IHC Counterstains Immunohistochemistry Antibodies and Proteins
IHC Troubleshooting Guide Immunohistochemistry Antibodies and Proteins
An Overview of Protein-Protein Interaction Analysis Protein-Protein Interactions Protein Isolation and Purification, Protein Modification and Crosslinking
Co-Immunoprecipitation (Co-IP) Protein-Protein Interactions Protein Isolation and Purification, Protein Modification and Crosslinking
Pull-Down Assays Protein-Protein Interactions Protein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking Protein Interaction Analysis Protein-Protein Interactions Protein Modification and Crosslinking, Protein Modification and Crosslinking
Label Transfer Protein Interaction Analysis Protein-Protein Interactions Protein Isolation and Purification, Protein Modification and Crosslinking
Far-Western Blot Analysis Protein-Protein Interactions Protein Isolation and Purification, Protein Gel Electrophoresis, Western Blotting,  Protein Modification and Crosslinking
An Overview of Protein-Nucleic Acid Interactions Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
Gel Shift Assays (EMSA) Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
Methods for Detecting Protein-DNA Interactions Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
Methods for Detecting Protein-RNA Interactions Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
Chromatin IP (ChIP Assays) Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
Methods for Labeling Nucleic Acids Protein-Nucleic Acid Interactions Protein Modification and Crosslinking
An Overview of Mass Spectrometry Protein Mass Spectrometry Analysis Protein Isolation and Purification, Protein Mass Spectrometry
Quantitative Proteomics Protein Mass Spectrometry Analysis Protein Isolation and Purification, Protein Mass Spectrometry
Sample Preparation for Mass Spectrometry Protein Mass Spectrometry Analysis Protein Isolation and Purification, Protein Mass Spectrometry
An Overview of Protein Labeling Protein Labeling Protein Isolation and Purification, Protein Modification and Crosslinking
Biotinylation Protein Labeling Protein Isolation and Purification, Protein Modification and Crosslinking
Metabolic Labeling and Chemoselective Ligation Protein Labeling Protein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Crosslinking and Protein Modification Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Chemistry of Crosslinking Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Crosslinking Applications Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Polyethylene Glycol (PEG) and Pegylation of Proteins Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Carbodiimide Crosslinker Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Amine-Reactive Crosslinker Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Sulfhydryl-Reactive Crosslinker Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Carbonyl-Reactive Crosslinker Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Photoreactive Crosslinker Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
Staudinger Ligation Reaction Chemistry Protein Crosslinking Protein Isolation and Purification, Protein Modification and Crosslinking
An Overview of Antibody Production and Purification Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Production (Immunogen Preparation) Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Isotyping and Characterization Methods Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Purification Methods Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody IgG Binding Proteins Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Fragmentation Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Structure and Classes Antibody Production and Purification Protein Expression, Antibodies and Proteins
Antibody Labeling and Immobilization Sites Antibody Production and Purification Protein Expression, Antibodies and Proteins
An Overview of Post-Translational Modification Post-Translational Modification Protein Expression
Phosphorylation Post-Translational Modification Protein Expression
Glycosylation Post-Translational Modification Protein Expression
An Overview of Protein Expression Systems Protein Expression Protein Expression
Cell-Free Protein Expression Protein Expression Protein Expression
Peptide Design Peptides Protein Expression
Peptide Synthesis Peptides Protein Expression
Amino Acid Physical Properties Peptides Protein Expression