The GeneArt® Synechococcus Protein Expression Kit is a second-generation Synechococcus cloning and expression system. Like the first-generation GeneArt® Synechococcus Engineering Kit, this expression system employs the cyanobacterium Synechococcus elongatus strain PCC 7942, but is optimized for high-level expression and offers dual protein tags for detection and/or purification of your gene of interest.
The kit includes frozen cyanobacterial cells, expression vector, OneShot® TOP10 Competent E. coli cells, and easy-to-follow protocols. Our Gibco® BG-11 Media, sold separately, is optimized for the growth and maintenance of select Cyanobacteria including S. elongatus.
- Express >10% total soluble protein from your gene of interest
- Detect and purify your protein of interest with 6His TEV and/or V5-His epitope tags
- Use (optional) seamless assembly to create your constructs
- Enables reliable results with exceptional strain viability and purity