Proven technology with quick, 5-minute TOPO® cloning procedure

经验证的TOPO®5分钟内快速克隆技术 

我们的Bac-to-Bac® 杆状病毒表达系统提供了一种快速、强力、可靠的方法来制备重组杆状病毒,从而在昆虫细胞中表达感兴趣的基因。经基因工程改造的pFastBac™载体与TOPO®平末端克隆技术结合在一起可将你的克隆反应简化至仅需5分钟的实验台反应,相比而言传统的限制性酶切克隆方法则需要花费大量时间,通常都需要2至3天。

Bac-to-Bac®表达系统的优势:

  • 滴度可高至10 8 pfu,可以进行大规模蛋白表达
  • pFastBac™ 载体含多角体启动子,可获得高得率的重组蛋白
  • 可靠快速的TOPO® 5分钟平末端克隆,节省时间
  • N端及C端添加的6xHis 标签可轻松进行纯化
  • 含有TEV 蛋白酶位点可用于去除N端及C端的标签从而得到天然蛋白质


下载Bac-to-Bac® 表达试剂盒手册

Advantages of the Bac-to-Bac® Expression Systems:

  • High titers up to 108 pfu enable large-scale protein expression
  • pFastBac™ vector contains polyhedrin promoter for high yields of recombinant protein
  • Reliable, rapid 5-minute blunt TOPO® cloning saves time
  • N- and C-Terminal 6xHis tag for easy purification
  • TEV protease sites enable removal of N- or C-terminal tag yielding a native protein

Download the Bac-to-Bac® Expression Kit Handbook

Bac-to-Bac® method delivers high titers

Traditional baculovirus systems require purification and amplification of an initial low-titer viral supernatant. In contrast, the Bac-to-Bac® Baculovirus Expression System is faster and easier because the technology relies on generation of recombinant baculovirus by site-specific transposition in E. coli rather than homologous recombination in insect cells, which often results in low titers.

Our pFastBac™ TOPO® Cloning vector recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid, which is then used for transfection into insect cells. After the very first transfection of the bacmid, you will produce high titers of pure recombinant baculovirus particles, saving weeks of precious time and enabling large-scale protein expression experiments.

Multiple kit formats for your convenience

We offer two formats of our Bac-to-Bac® Baculovirus Expression Systems. The Expression system format in the most complete kit available; these kits include pFastBac™ TOPO® cloning vector, fast competent cells for cloning, specialized competent cells bacmid generation and our CellFectin® II Transfection reagent. If you simply need the pFastBac™ vector, we offer a Cloning kit format, which contain the pFastBac™ TOPO® vector and competent cells for cloning in the gene of interest.