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哺乳动物瞬时表达系统可对蛋白质进行灵活快速的制备。它非常适用于人或其他哺乳动物蛋白的表达,因为这些系统相比其他基于大肠杆菌、酵母、或昆虫细胞宿主的表达系统,可制得具有更天然折叠和翻译后修饰(如糖基化)的重组蛋白。

Choose the mammalian transient system that's right for you

  The most widely used transient expression system Gram per liter protein yield 293-based system Up to 3 g/L protein yield CHO-based system Most-cost effective
  FreeStyle™ 293 System Expi293™ System ExpiCHO™ System
Cells FreeStyle™ 293-F Expi293-F™ ExpiCHO-S™
Media FreeStyle™ 293 Medium Expi293™ Medium ExpiCHO™ Medium
Transfection reagent 293Fectin™ ExpiFectamine™ 293 Transfection Kit ExpiFectamine™ CHO Transfection Kit
Positive control vector pCMV•Sport-βgal Antibody-expressing vector Antibody-expressing vector
Maximum working culture Density 2 x 106/mL 6 x 106/mL 10 x 106/mL
Cell density for transfection 1 x 106/mL 2.5 x 106/mL 6 x 106/mL
Protein yield* 20–200 mg/L Up to 1 g/L Up to 3 g/L
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*Typical yield of human IgGs