Proteases and Protein-Cleaving Reagents

We offer purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis, and polypeptide structural characterization.

For protein digestion using mass spectrometry, we offer a wide range of highly purified and MS-validated proteases.

Choose the right protease for your application

Enzyme Purified protease Immobilized proteases Specificity
Factor Xa 32521
32520
N/A Cleaves at the carboxyl side of Ile-Glu-Gly-Arg sequence
Human Rhinovirus 3C Protease
(HRV 3C)
88946 N/A Highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue
S. aureus V-8 Protease 20195 20151 Cleaves at the carboxyl side of aspartate and glutamate residues
Enzyme Purified protease Immobilized proteases Specificity
Ficin N/A 44881 Cleaves non-specifically at exposed residues, favors aromatic residues
Papain N/A 20341 Cleaves nonspecifically at exposed residues
Pepsin N/A 20343 Cleaves nonspecifically at exposed residues, favors aromatic residues
Enzyme Purified protease Immobilized proteases Specificity
Trypsin, MS Grade 90057
90058
90059
90305
N/A Cleaves at the carboxyl side of arginine and lysine residues
Lys-C Protease, MS Grade 90051 N/A Cleaves at the carboxyl side of lysine residues
Trypsin/Lys-C Protease Mix, MS Grade A41007 N/A Cleaves at the carboxyl side of lysine residues
Lys-N Protease, MS Grade 90300
90301
N/A Cleaves at the amino side of lysine residues
Asp-N Protease, MS Grade 90053 N/A Cleaves at the amino side of aspartate residues
Chymotrypsin, MS Grade 90056 N/A Cleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan, and leucine
Glu-C Protease, MS Grade 90054 N/A Cleaves at the carboxyl side of glutamate or aspartate
Enzyme Purified protease Immobilized proteases Specificity
Trypsin (TPCK) 20233 20230 Cleaves at the carboxyl side of arginine and lysine residues
Enzyme Purified protease Immobilized proteases Specificity
Proteinase K 17916 N/A Cleaves at the carboxyl side of aliphatic, aromatic, and hydrophobic residues