The Thermo Scientific Pierce Standards are designed for a variety of applications, including sensitivity assessment, determination of digestion efficiency, chromatography assessment and/or as controls for sample analysis. Standards range from single component formulations to complex human-based digests. Each standard is provided with a Certificate of Analysis.

Thermo Scientific Pierce标准品专为广泛应用而设计,包括灵敏度评估、消化效率测定、色谱评估、和/或作为样品分析对照。产品范围涵盖了从单组分制剂到复杂的基于人体的消化物,且都附有质量分析证书。
  • 更多选择——各种不同的标准品,能够满足广泛的应用/需求
  • 稳定——在建议温度下,可保存超过一年的时间
  • 确保验证——所有标准品均由ISO 9001设备制造,并通过Thermo Scientific质谱仪检测和全面测试

为您的应用选择合适标准品

 
  Pierce BSA Digest Standard Pierce 6-Protein Mixture Standard Pierce HeLa Protein Digest Standard Pierce TMT11plex Yeast Digest Standard
Components
(abbreviated)
BSA tryptic peptides Lysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species) HeLa lysate peptides Yeast lysate peptide digest
Format Lyophilized Lyophilized Lyophilized Lyophilized
Primary application Protein sample control Chromatography assessment Complex sample control Complex sample for TMT-labeling
Complexity of standard ++ +++ ++++ +++
Recommended storage -20°C -20°C -20°C -20°C
  Order Order Order Order
 
  Pierce Reserpine Standard for LC-MS
Components
(abbreviated)
Reserpine molecule
Format Liquid
Primary application Sensitivity assessment
Complexity of standard +
Recommended storage 4°C
  Order
 
  Pierce Peptide Retention Time Calibration Mixture Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix)
Components
(abbreviated)
15 heavy tryptic peptides (yeast) 7 yeast tryptic peptides (5 variations of light or heavy amino acids)
Format Frozen liquid Frozen liquid
Primary application Chromatography assessment Targeted MS Assay control
Complexity of standard

++

++
Recommended storage -80°C -80°C
  Order Order
 
  Pierce Intact Protein Standard Mix
Components
(abbreviated)
IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
Format Lyophilized
Primary application Top down method development
Complexity of standard +++
Recommended storage -20°C
  Order
 
  Pierce BSA Digest Standard Pierce 6-Protein Mixture Standard Pierce HeLa Protein Digest Standard Pierce TMT11plex Yeast Digest Standard
Components
(abbreviated)
BSA tryptic peptides Lysozyme, BSA, Cytochrome C, Alcohol dehyrogenase, B-galactosidase, Apotransferrin (multi-species) HeLa lysate peptides Yeast lysate peptide digest
Format Lyophilized Lyophilized Lyophilized Lyophilized
Primary application Protein sample control Chromatography assessment Complex sample control Complex sample for TMT-labeling
Complexity of standard ++ +++ ++++ +++
Recommended storage -20°C -20°C -20°C -20°C
  Order Order Order Order
 
  Pierce Reserpine Standard for LC-MS
Components
(abbreviated)
Reserpine molecule
Format Liquid
Primary application Sensitivity assessment
Complexity of standard +
Recommended storage 4°C
  Order
 
  Pierce Peptide Retention Time Calibration Mixture Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix)
Components
(abbreviated)
15 heavy tryptic peptides (yeast) 7 yeast tryptic peptides (5 variations of light or heavy amino acids)
Format Frozen liquid Frozen liquid
Primary application Chromatography assessment Targeted MS Assay control
Complexity of standard

++

++
Recommended storage -80°C -80°C
  Order Order
 
  Pierce Intact Protein Standard Mix
Components
(abbreviated)
IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
Format Lyophilized
Primary application Top down method development
Complexity of standard +++
Recommended storage -20°C
  Order
 
  Pierce 利血平LC-MS标准品 Pierce 肽保留时间校准混合物 Pierce BSA 消化标准品 Pierce 6种蛋白混合标准品 Pierce完整蛋白标准品混合物 Pierce HeLa 蛋白消化标准品
组份
(缩写)
利血平分子 15个重胰蛋白酶肽(酵母) BSA胰蛋白酶肽 溶菌酶、BSA、细胞色素C、醇脱氢酶、B—半乳糖苷酶、脱铁运铁蛋白(多物种来源) IGF-I LR3、硫氧还蛋白、G蛋白、碳酸酐酶II、AG蛋白(嵌合)、ExoKlenow HeLa裂解肽
规格 液体 冷冻液体 冻干粉 冻干粉 冻干粉 冻干粉
主要应用 灵敏度评估 色谱评估 蛋白样品对照 色谱评估 自顶向下方法开发(Top down) 复杂样品对照
标准品复杂度 +

++

++ +++ +++ ++++
建议保存条件 4°C -80°C -20°C -20°C -20°C -20°C
  订购 订购 订购 订购 订购 订购

需要检测消化效率的标准品?

Thermo Scientific Pierce消化指示剂是一种单一的非哺乳动物重组蛋白(26kDa),含有5个特征肽,可用于测定多个样品的消化效率和可重复性。蛋白序列和推荐用于监测样品的多肽详见产品说明书中。

性能数据

Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.

在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。

Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.

Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。

Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.

在Thermo Scientific™ Vantage™三重四极杆质谱仪上500fg利血平的选择反应监测(SRM)示例。 在Hypersil GOLD aQ 2.1x20 mm Javelin色谱柱样品环上进行3次5μL,100 fg /μL Pierce™利血平进样的等量色谱图(70%甲醇300μL/ min 流速等度洗脱):30%水,0.05%甲酸(上图)。监测的SRM跃迁分别为609.3-195.1和609.3-448.2 m/z,峰宽0.2 FWHM Q1(下图)。

Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.

Thermo Scientific Pierce HeLa 消化标准品基峰色谱图。200 ng Pierce HeLa 蛋白消化标准品色谱图。 使用Thermo Scientific™ Acclaim™ PepMap™ 100 3μmx 75μmx 15 cm色谱柱(产品号:160321),以2-35%梯度(A:溶于水中的0.1% FA ,B:溶于100%乙腈中的0.1%FA),在300nL /分钟条件下,处理120分钟,并在Thermo Scientific™ LTQ Orbitrap™ XL质谱仪上进行检测。

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